Dual-Wavelength Sensor System for Fluorescence Detection of Bacillus subtilis Endospores

IF 3.2 4区 工程技术 Q2 CHEMISTRY, MULTIDISCIPLINARY
Bonghyun Jo, Youngwon Ju, Hee-Won Shin, Joohoon Kim, You Jin Jeong, Tae Kyu Ahn, Hyun Suk Jung
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引用次数: 0

Abstract

Biological threats to military and civilian sectors underscore the need for compact, cost-effective, and durable sensor systems capable of the sensitive and selective detection of bio-threat agents. Fluorescence-based techniques, particularly those employing UV light, have proven effective for detecting biological fluorophores such as proteins and cofactors. However, conventional laser-induced fluorescence (LIF) systems, while highly sensitive, are bulky, expensive, and require significant power and maintenance. To address these limitations, light-emitting diodes (LEDs) have emerged as a promising alternative, offering compact, robust, and low-maintenance solutions. Recent advancements in UV LEDs, spanning 200–400 nm, align with the excitation of natural fluorophores found in bacteria, spores, and viruses, enabling the development of practical fluorescence sensors. In this study, we developed a dual-wavelength fluorescence sensor system employing UV LEDs at 280 nm and 365 nm for the selective detection of Bacillus subtilis endospores, a surrogate for the pathogenic Bacillus anthracis. The system integrates optimized optical lenses and a bio-cell utilizing replaceable quartz sample tubes to minimize contamination and enhance usability. The sensor demonstrated selective detection at concentrations as low as 107 spores/mL. This portable, cost-effective system provides a practical solution for rapid and reliable detection of biological threats, meeting critical field requirements for size, weight, and durability.

枯草芽孢杆菌内生孢子荧光检测的双波长传感器系统
对军事和民用部门的生物威胁强调需要紧凑、具有成本效益和耐用的传感器系统,能够敏感和选择性地检测生物威胁剂。基于荧光的技术,特别是使用紫外线的技术,已被证明对检测生物荧光团(如蛋白质和辅助因子)是有效的。然而,传统的激光诱导荧光(LIF)系统虽然高度敏感,但体积庞大,价格昂贵,并且需要大量的电力和维护。为了解决这些限制,发光二极管(led)作为一种有前途的替代方案出现了,它提供了紧凑、坚固和低维护的解决方案。UV led的最新进展,跨越200 - 400nm,与细菌,孢子和病毒中发现的天然荧光团的激发一致,使实用荧光传感器的开发成为可能。在这项研究中,我们开发了一种双波长荧光传感器系统,采用紫外led在280 nm和365 nm选择性检测枯草芽孢杆菌内生孢子,致病性炭疽芽孢杆菌的替代品。该系统集成了优化的光学透镜和利用可更换的石英样管的生物电池,以最大限度地减少污染并提高可用性。该传感器在低至107孢子/mL的浓度下具有选择性检测。这种便携、经济高效的系统为快速、可靠地检测生物威胁提供了实用的解决方案,满足了对尺寸、重量和耐用性的关键现场要求。
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来源期刊
Korean Journal of Chemical Engineering
Korean Journal of Chemical Engineering 工程技术-工程:化工
CiteScore
4.60
自引率
11.10%
发文量
310
审稿时长
4.7 months
期刊介绍: The Korean Journal of Chemical Engineering provides a global forum for the dissemination of research in chemical engineering. The Journal publishes significant research results obtained in the Asia-Pacific region, and simultaneously introduces recent technical progress made in other areas of the world to this region. Submitted research papers must be of potential industrial significance and specifically concerned with chemical engineering. The editors will give preference to papers having a clearly stated practical scope and applicability in the areas of chemical engineering, and to those where new theoretical concepts are supported by new experimental details. The Journal also regularly publishes featured reviews on emerging and industrially important subjects of chemical engineering as well as selected papers presented at international conferences on the subjects.
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