Vitex leucoxylon extract conjugated CMC membrane for bone regeneration, wound healing, and anticancer applications

IF 2.5 4区化学 Q2 Engineering

Chemical Papers Pub Date : 2025-06-18 DOI:10.1007/s11696-025-04125-1

Nayef Abdulaziz Aldabaan

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引用次数: 0

Abstract

Phytochemicals have gained importance due to their diverse applications in the biomedical and pharmacological fields. The present study was designed to evaluate the biomedical applications and characterization of Vitex leucoxylon extract conjugated CMC membranes. Phyto-conjugated CMC membranes were prepared through solvent casting technique and characterized using FTIR, XRD, SEM, EDX and contact angle analysis. Antioxidant activity was performed using DPPH assay; inflammatory marker study was conducted based on COX-2 inhibition study; hemocompatibility nature was evaluated using hemolysis; cytotoxicity and anticancer activity against triple negative breast cancer MDA-MB231 cell line was screened using MTT assay; and bone cell regeneration study was conducted using MG-63 cell line. Phyto-conjugated CMC membranes showed the presence of secondary metabolites which was confirmed by FTIR analysis. Surface morphology and EDX analysis confirmed conjugation of plant extract within the membranes. XRD revealed the change in the nature of the phyto-conjugated membrane with changes in the peak values. Wettability nature was revealed by the contact angle. Physical properties such as water vapor transmission rate (0.670%), moisture retention (92.60%), and swelling ability (68.76%) revealed optimum characteristics of the membrane. In vitro studies showed significant antioxidant activity and COX-2 inhibition. The phyto-conjugated CMC membranes showed excellent hemocompatibility and biocompatibility. The anticancer activity was confirmed by the cytotoxicity results (IC₅₀ = 157.74 µg/mL). Moreover, in vitro bone cell regeneration and migration assay results illustrated prominent bone cell regeneration (87.88%) and cell migration characteristics. These findings open new avenues for the development of advanced biomaterials, significantly enhancing the efficacy of bone tissue regeneration, wound healing, and targeted anticancer therapies.

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白荆提取物偶联CMC膜用于骨再生、伤口愈合和抗癌

植物化学物质由于其在生物医学和药理学领域的广泛应用而变得越来越重要。本研究旨在评价白荆提取物偶联CMC膜的生物医学应用及性能。采用溶剂铸造法制备了植物共轭CMC膜，并用FTIR、XRD、SEM、EDX和接触角分析对其进行了表征。DPPH法测定抗氧化活性；在COX-2抑制研究的基础上进行炎症标志物研究；采用溶血法评价血液相容性；采用MTT法筛选三阴性乳腺癌MDA-MB231细胞株的细胞毒性和抗癌活性；用MG-63细胞系进行骨细胞再生研究。FTIR分析证实，植物偶联CMC膜存在次生代谢产物。表面形貌和EDX分析证实了膜内植物提取物的偶联。x射线衍射（XRD）显示了植物共轭膜性质的变化和峰值的变化。通过接触角揭示其润湿性。水蒸气透过率（0.670%）、保湿性（92.60%）和溶胀性（68.76%）等物理性能显示了膜的最佳特性。体外研究显示出显著的抗氧化活性和COX-2抑制作用。植物偶联CMC膜具有良好的血液相容性和生物相容性。细胞毒性（IC50 = 157.74µg/mL）证实了其抗癌活性。此外，体外骨细胞再生和迁移实验结果显示出显著的骨细胞再生（87.88%）和细胞迁移特征。这些发现为先进生物材料的发展开辟了新的途径，显著提高了骨组织再生、伤口愈合和靶向抗癌治疗的功效。

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来源期刊

Chemical Papers Chemical Engineering-General Chemical Engineering

CiteScore

3.30

自引率

4.50%

发文量

590

期刊介绍： Chemical Papers is a peer-reviewed, international journal devoted to basic and applied chemical research. It has a broad scope covering the chemical sciences, but favors interdisciplinary research and studies that bring chemistry together with other disciplines.