Structural Characteristics and Antioxidant Activity of Two Novel Water-Soluble Polysaccharides from Acanthopanax leucorrhizus

IF 0.9 4区化学 Q4 CHEMISTRY, MEDICINAL

Chemistry of Natural Compounds Pub Date : 2025-07-19 DOI:10.1007/s10600-025-04727-x

H. B. Hu, Z. Y. Cheng, M. H. Han, H. P. Liang, Y. Wu, H. S. Hu, L. L. Zhang

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Abstract

Two novel water-soluble polysaccharides (ALPS-21 and ALPS-32) were isolated and purified from Acanthopanax leucorrhizus. Their structures were investigated by HPGPC, HPLC, IR, methylation and NMR, and the antioxidant activity was evaluated in vitro by 1,1-diphenyl-2-picrylhydrazyl radical (DPPH^·), hydroxyl radical (HO^·), and 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS^·+) assay. Results showed that ALPS-21 was a 4.36 kDa neutral heteropolysaccharide composed of glucose, galactose, mannose, xylose in a ratio of 3.5:1.3:0.9:2.1, whereas ALPS-32 was a 5.78 kDa acidic heteropolysaccharide composed of glucose, galactose, mannose, and glucopyranuronic acid in a ratio of 4.5:1.3:1.1:1.0. Moreover, ALPS-21 and ALPS-32 can scavenge DPPH^·, HO^·, and ABTS^·+ in a dose-dependent manner, suggesting that ALPS-21 and ALPS-32 may be promising as a potential natural antioxidant.

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两种新型白沙五加水溶性多糖的结构特征及抗氧化活性研究

从白根棘五加中分离纯化了两种新的水溶性多糖（ALPS-21和ALPS-32）。采用HPGPC、HPLC、IR、甲基化和NMR等方法对其结构进行了表征，并采用1,1-二苯基-2-吡啶肼基自由基（DPPH·）、羟基自由基（HO·）和2,2′-氮化喹啉-（3-乙基苯并噻唑-6-磺酸）（ABTS·+）法对其体外抗氧化活性进行了评价。结果表明，ALPS-21是由葡萄糖、半乳糖、甘露糖、木糖按3.5:1.3:0.9:2.1的比例组成的4.36 kDa的中性杂多糖，而ALPS-32是由葡萄糖、半乳糖、甘露糖、葡萄糖醛酸按4.5:1.3:1.1:1.0的比例组成的5.78 kDa的酸性杂多糖。此外，ALPS-21和ALPS-32还能以剂量依赖的方式清除DPPH·、HO·和ABTS·+，这表明ALPS-21和ALPS-32可能是一种潜在的天然抗氧化剂。

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来源期刊

Chemistry of Natural Compounds 化学-有机化学

CiteScore

1.40

自引率

25.00%

发文量

265

审稿时长

7.8 months

期刊介绍： Chemistry of Natural Compounds publishes reviews and general articles about the structure of different classes of natural compounds, the chemical characteristics of botanical families, genus, and species, to establish the comparative laws and connection between physiological activity and the structure of substances.