Determining the Active Ingredients of Dendrobium officinale and Tracing Its Region of Origin

IF 3 3区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Food Analytical Methods Pub Date : 2025-06-17 DOI:10.1007/s12161-025-02824-9

Jiao Zhang, An Wang, Xiao Wei, Zhenhuan Liu, Zhengxu Hu, Jing Wang, Xinglian Chen, Lijuan Du, Hongcheng Liu, Tao Lin

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Abstract

A method for the determination of 22 active ingredients, including flavonoids, phenols, and glycosides, in Dendrobium officinale was established using UHPLC–MS/MS. The 22 active compounds presented good linear correlation coefficients in the concentration range of 0.005 and 10 µg·mL⁻¹, limits of detection and quantification ranging between 0.008 and 2.25 mg·kg⁻¹ and 0.025 and 7.5 mg·kg⁻¹, respectively, and interday and intraday RSDs ranging between 0.14 and 12.57% and 0.08 and 9.92%, respectively, and RE values ranging between 0.01 and 9.57% and 0.02 and 9.33%, respectively. Moreover, the detection sensitivity and stability of the method were good. D. officinale samples from different regions in Yunnan (Xishuangbanna, Baoshan, Dehong, and Wenshan) were effectively distinguished using OPLS-DA on the basis of the contents of 22 compounds, and ten differentially abundant metabolites, such as syringin, scutellarein, gallic acid, and gentisic acid, were identified, which can be used to identify the origin of D. officinale to some extent. The samples from Dehong presented relatively high levels of syringin, gentisic acid, protocatechuic acid, and gallic acid; the samples from Baoshan contained relatively high levels of apigenin, naringin, and 4-hydroxybenzaldehyde; the samples from Wenshan exhibited relatively high levels of epicatechin gallate, scopoletin, and scutellarein; and the samples from Xishuangbanna presented relatively low levels of protocatechuic acid and gallic acid. The accumulation of active ingredients was affected by the D. officinale growth environment. There were no significant differences in the contents of epicatechin gallate, scopoletin, or scutellarein in the samples from the Dehong, Baoshan, and Xishuangbanna regions or in the content of apigenin in the samples from the Dehong and Xishuangbanna regions; however, there were large significant differences in the contents of all other differentially abundant metabolites between samples from the Wenshan region and those from the other three regions. This method is simple and effective and can provide technical support to trace the origin of Dendrobium species and other agricultural products.

Graphical Abstract

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铁皮石斛有效成分测定及产地溯源

建立了hplc - MS/MS法测定铁皮石斛中黄酮类、酚类、苷类22种有效成分含量的方法。22种活性化合物在0.005 ~ 10µg·mL−1的浓度范围内呈良好的线性相关，检测限和定量限分别为0.008 ~ 2.25 mg·kg−1和0.025 ~ 7.5 mg·kg−1，日内和日间rsd分别为0.14 ~ 12.57%和0.08 ~ 9.92%，RE分别为0.01 ~ 9.57%和0.02 ~ 9.33%。该方法具有良好的检测灵敏度和稳定性。利用OPLS-DA对云南不同地区（西双版纳、宝山、德宏、文山）药材样品中22个化合物的含量进行了有效区分，鉴定出紫丁香苷、黄芩苷、没食子酸、龙胆酸等10个差异丰度的代谢物，可在一定程度上用于鉴别药材来源。德宏样品中紫丁香苷、龙胆酸、原儿茶酸和没食子酸含量较高；宝山样品中芹菜素、柚皮苷和4-羟基苯甲醛含量较高；文山样品中表儿茶素、没食子酸酯、东莨菪碱和灯盏花苷含量较高；西双版纳样品中原儿茶酸和没食子酸含量相对较低。有效成分的积累受生长环境的影响。德宏、宝山和西双版纳地区样品中表儿茶素、没食子酸酯、东莨菪素和花楸素的含量以及芹菜素的含量没有显著差异；然而，文山地区与其他三个地区的样品在所有其他差异丰度代谢物的含量上存在显著差异。该方法简便有效，可为石斛等农产品溯源提供技术支持。图形抽象

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来源期刊

Food Analytical Methods 农林科学-食品科技

CiteScore

6.00

自引率

3.40%

发文量

244

审稿时长

3.1 months

期刊介绍： Food Analytical Methods publishes original articles, review articles, and notes on novel and/or state-of-the-art analytical methods or issues to be solved, as well as significant improvements or interesting applications to existing methods. These include analytical technology and methodology for food microbial contaminants, food chemistry and toxicology, food quality, food authenticity and food traceability. The journal covers fundamental and specific aspects of the development, optimization, and practical implementation in routine laboratories, and validation of food analytical methods for the monitoring of food safety and quality.