Simultaneous Determination of Flupyradifurone and Its Metabolites in Animal-Derived Foods Using QuEChERS-UPLC-MS/MS

IF 3 3区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Food Analytical Methods Pub Date : 2025-06-18 DOI:10.1007/s12161-025-02837-4

Xiao Hu, Ying Mei, Xianjun Li, Yan Chen

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引用次数: 0

Abstract

In this research, an analytical method for the simultaneous determination of flupyradifurone (FLU) and its three principal metabolites (difluoroethylamino-furanone (DFEAF), 6-chloronicotinic acid (6-CNA), and difluoroacetic acid (DFA)) in animal-derived food matrices was established based on the QuEChERS and ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). A low sample injection volume (3 µL) and dilution strategy were applied, without the use of isotope internal standards or matrix-matched calibration curves to compensate for the matrix effects. Recoveries for all matrix-matched samples at three spiked levels (n = 6) were ranged from 69.9% to 114.0% with relative standard deviations (RSDs) of 0.8%–10.4%. The method demonstrated excellent linearity (correlation coefficients R > 0.998), extremely low limits of quantitations (0.001–0.025 mg/kg), and acceptable matrix effects (ranged from − 12.7% to 13.4%). The validated method provides a reliable tool for monitoring FLU and its metabolite residues in practical sample analysis, including livestock and poultry meat, viscera, milk, and honey, which is essential to risk assessment, compliance with good agricultural practices and regulatory oversight.

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QuEChERS-UPLC-MS/MS同时测定动物性食品中氟吡地酮及其代谢物

本研究建立了基于QuEChERS和超高效液相色谱-串联质谱（UPLC-MS/MS）同时测定动物源性食品基质中氟吡地酮（FLU）及其3种主要代谢物(二氟乙基氨基呋喃酮（DFEAF）、6-氯烟酸（6-CNA）和二氟乙酸（DFA）的分析方法。采用低进样量（3µL）和稀释策略，不使用同位素内标或基质匹配校准曲线来补偿基质效应。在3个加标水平（n = 6）下，加标回收率为69.9% ~ 114.0%，相对标准偏差（rsd）为0.8% ~ 10.4%。该方法具有良好的线性关系（相关系数R >； 0.998），极低的定量限（0.001 ~ 0.025 mg/kg）和可接受的基质效应（范围为- 12.7% ~ 13.4%）。经过验证的方法为在实际样品分析中监测流感及其代谢物残留提供了可靠的工具，包括牲畜和家禽肉、内脏、牛奶和蜂蜜，这对风险评估、遵守良好农业规范和监管监督至关重要。

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来源期刊

Food Analytical Methods 农林科学-食品科技

CiteScore

6.00

自引率

3.40%

发文量

244

审稿时长

3.1 months

期刊介绍： Food Analytical Methods publishes original articles, review articles, and notes on novel and/or state-of-the-art analytical methods or issues to be solved, as well as significant improvements or interesting applications to existing methods. These include analytical technology and methodology for food microbial contaminants, food chemistry and toxicology, food quality, food authenticity and food traceability. The journal covers fundamental and specific aspects of the development, optimization, and practical implementation in routine laboratories, and validation of food analytical methods for the monitoring of food safety and quality.