Development and Validation of a Miniaturized Two-Step Neutral and Acidified Acetonitrile Extraction Method for Simultaneous Determination of Pesticides, Veterinary Drugs, and Mycotoxins in Eggs by UHPLC-MS/MS

IF 3 3区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Food Analytical Methods Pub Date : 2025-04-16 DOI:10.1007/s12161-025-02800-3

Yuri Pereira de Souza, Angélica Castanheira de Oliveira, Lucia Helena Pinto Bastos, André Victor Sartori, Bernardete Ferraz Spisso

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引用次数: 0

Abstract

This study aimed to develop and validate an attractive two-step neutral and acidified acetonitrile extraction method for the simultaneous determination of pesticides, veterinary drugs, and mycotoxins in eggs by UHPLC-MS/MS. A simple miniaturized solvent extraction method followed by extract concentration was proposed for sample treatment without the need for subsequent extract cleanup steps. Satisfactory recoveries (70 to 120%), with relative standard deviations below lower than 22%, were achieved for 285 compounds. The limits of quantification ranged from 0.1 to 50 µg kg⁻¹. Calibration plots with R² ≥ 0.99 were obtained for all analytes. The validated method was successfully employed for the analysis of egg samples collected from local supermarkets in Rio de Janeiro, Brazil. The results obtained demonstrate its high-throughput and suitability for routine analysis.

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UHPLC-MS/MS同时测定鸡蛋中农药、兽药和真菌毒素的微型两步中性酸化乙腈萃取方法的建立与验证

本研究旨在建立并验证一种两步中性酸化乙腈萃取方法，用于UHPLC-MS/MS同时测定鸡蛋中的农药、兽药和真菌毒素。提出了一种简单的小型溶剂萃取法，然后是萃取物浓缩法，用于样品处理，而不需要后续的萃取物清理步骤。285个化合物的加样回收率为70% ~ 120%，相对标准偏差小于22%。定量限为0.1 ~ 50µg kg−1。所有分析物均获得R2≥0.99的校准图。该方法已成功地应用于巴西里约热内卢当地超市的鸡蛋样品分析。结果表明，该方法具有较高的通量，适用于常规分析。

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来源期刊

Food Analytical Methods 农林科学-食品科技

CiteScore

6.00

自引率

3.40%

发文量

244

审稿时长

3.1 months

期刊介绍： Food Analytical Methods publishes original articles, review articles, and notes on novel and/or state-of-the-art analytical methods or issues to be solved, as well as significant improvements or interesting applications to existing methods. These include analytical technology and methodology for food microbial contaminants, food chemistry and toxicology, food quality, food authenticity and food traceability. The journal covers fundamental and specific aspects of the development, optimization, and practical implementation in routine laboratories, and validation of food analytical methods for the monitoring of food safety and quality.