Bacterial microenvironment-responsive Fe-Ce6 nanoparticles accelerate infected wound healing via in situ generation of nanozyme and photodynamic antibacterial activity

IF 7.4 2区材料科学 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY

Science China Materials Pub Date : 2025-07-15 DOI:10.1007/s40843-025-3459-7

Zehua Wang (, ), Yongliang Hao (, ), Hongxue Li (, ), Huanyi Liang (, ), Xiaokuang Xue (, ), Tiejin Chen (, ), Yiying Wang (, ), Jian Li (, ), Jiechao Ge (, ), Pengfei Wang (, )

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引用次数: 0

Abstract

Bacterial infection poses a significant challenge in clinical wound management. Traditional antibiotic therapies are hampered by cytotoxicity and the emergence of drug resistance. However, current photodynamic therapy (PDT) and nanozyme-based antibacterial strategies often lack microenvironment specificity, exhibiting persistent activity that risks tissue damage. To overcome these limitations, we developed bacterial microenvironment responsive Fe-Ce6 nanoparticles (NPs) for in situ generation of peroxidase (POD)-like activity and PDT activation to enhance antibacterial wound therapy. Under bacteria-secreted ATP stimulation, Fe-Ce6 NPs disassembled and in situ formed Fe-ATP complexes, while synchronously releasing the Ce6 photosensitizer. The Fe-ATP complex with POD like activity converts H₂O₂ into hydroxyl radicals (·OH), and Ce6 generates singlet oxygen (¹O₂) under 671 nm laser irradiation, synergistically enhancing nanozyme-PDT antibacterial effects. The intracellular ATP released from lysed bacteria further amplifies this cascade, promoting the formation of Fe-ATP complex and the release of Ce6, ultimately inducing an “avalanche effect”, efficiently killing bacteria and reinforcing therapeutic action. In vitro, the system demonstrates remarkable antibacterial activity against S. aureus and E. coli in simulated bacterial environments. In vivo, it exhibits substantial bactericidal efficacy and accelerates wound healing. This study presents the Fe-Ce6 NPs smart system activated by bacterial microenvironments via an “off-on” mechanism, enabling precise reactive oxygen species generation control, significantly reducing non-target tissue damage associated with traditional therapies, and offering a novel paradigm for developing microenvironment-responsive intelligent antibacterial systems.

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细菌微环境响应的Fe-Ce6纳米颗粒通过原位产生纳米酶和光动力抗菌活性加速感染伤口愈合

细菌感染对临床伤口管理提出了重大挑战。传统的抗生素治疗受到细胞毒性和耐药性出现的阻碍。然而，目前的光动力疗法（PDT）和基于纳米酶的抗菌策略往往缺乏微环境特异性，表现出持续的活性，可能导致组织损伤。为了克服这些限制，我们开发了细菌微环境响应的Fe-Ce6纳米颗粒（NPs），用于原位产生过氧化物酶（POD）样活性和PDT激活，以增强抗菌伤口治疗。在细菌分泌的ATP刺激下，Fe-Ce6 NPs分解并原位形成Fe-ATP复合物，同时释放Ce6光敏剂。具有POD样活性的Fe-ATP配合物在671nm激光照射下将H2O2转化为羟基自由基（·OH）， Ce6生成单线态氧（1O2），协同增强纳米酶- pdt抗菌效果。从裂解细菌中释放的细胞内ATP进一步放大了这一级联反应，促进Fe-ATP复合物的形成和Ce6的释放，最终诱导“雪崩效应”，有效地杀死细菌并加强治疗作用。在体外，该系统在模拟细菌环境中对金黄色葡萄球菌和大肠杆菌表现出显著的抗菌活性。在体内，它显示出大量的杀菌功效，并加速伤口愈合。本研究提出了由细菌微环境通过“开关”机制激活的Fe-Ce6 NPs智能系统，实现了精确的活性氧生成控制，显着减少了传统治疗相关的非靶向组织损伤，并为开发微环境响应型智能抗菌系统提供了新的范例。

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来源期刊

Science China Materials Materials Science-General Materials Science

CiteScore

11.40

自引率

7.40%

发文量

949

期刊介绍： Science China Materials (SCM) is a globally peer-reviewed journal that covers all facets of materials science. It is supervised by the Chinese Academy of Sciences and co-sponsored by the Chinese Academy of Sciences and the National Natural Science Foundation of China. The journal is jointly published monthly in both printed and electronic forms by Science China Press and Springer. The aim of SCM is to encourage communication of high-quality, innovative research results at the cutting-edge interface of materials science with chemistry, physics, biology, and engineering. It focuses on breakthroughs from around the world and aims to become a world-leading academic journal for materials science.