Microfluidic synthesis of phosphatidylcholine liposomes for verbascoside delivery into C2C12 cells

IF 2.6 4区材料科学 Q3 CHEMISTRY, MULTIDISCIPLINARY

Journal of Nanoparticle Research Pub Date : 2025-06-16 DOI:10.1007/s11051-025-06365-4

Laura Chronopoulou, Roya Binaymotlagh, Manuela Bozzi, Marisa Colone, Anna Rita Stringaro, Francesca Sciandra, Cleofe Palocci

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引用次数: 0

Abstract

The interest of lipid vesicles for applications in the pharmaceutical field is increasing, especially for preparing drug and gene delivery vectors. There are different methods for the preparation of these vesicles, however, microfluidic-based methods provide significant advantages over other synthetic protocols like extrusion and sonication. In this study, monodisperse liposomes based on L-α-phosphatidylcholine were synthesized using a versatile capillary hydrodynamic flow-focusing device to deliver verbascoside into murine C2C12 muscle cells. The size and surface charge of the obtained liposomes were studied using Dynamic Light Scattering (DLS) and zeta-potential measurements. TEM and SAXS analyses were used to investigate the shape and lamellarity of the structures. By introducing a suitable dye into the phospholipid membrane of the liposomes and using confocal fluorescence microscopic analysis, liposomes internalization in C2C12 cells was confirmed in vitro. In addition, verbascoside encapsulation in the vesicles protected it efficiently, increasing its antioxidant activity against ROS production in C2C12 cells.

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微流控合成毛蕊花糖苷向C2C12细胞传递的磷脂酰胆碱脂质体

脂质囊泡在制药领域的应用越来越受到关注，特别是在制备药物和基因传递载体方面。制备这些囊泡有不同的方法，然而，基于微流体的方法比其他合成方案（如挤出和超声）具有显著的优势。本研究采用多用途毛细管流体动力聚焦装置合成L-α-磷脂酰胆碱单分散脂质体，将毛蕊花苷输送到小鼠C2C12肌细胞中。利用动态光散射（DLS）和ζ电位测量研究了所得脂质体的大小和表面电荷。利用TEM和SAXS分析研究了结构的形状和层状。通过在脂质体磷脂膜中引入合适的染料，利用共聚焦荧光显微镜分析，证实脂质体在体外C2C12细胞内化。此外，毛蕊花苷在囊泡中的包封有效地保护了它，增加了它对C2C12细胞ROS产生的抗氧化活性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Nanoparticle Research 工程技术-材料科学：综合

CiteScore

4.40

自引率

4.00%

发文量

198

审稿时长

3.9 months

期刊介绍： The objective of the Journal of Nanoparticle Research is to disseminate knowledge of the physical, chemical and biological phenomena and processes in structures that have at least one lengthscale ranging from molecular to approximately 100 nm (or submicron in some situations), and exhibit improved and novel properties that are a direct result of their small size. Nanoparticle research is a key component of nanoscience, nanoengineering and nanotechnology. The focus of the Journal is on the specific concepts, properties, phenomena, and processes related to particles, tubes, layers, macromolecules, clusters and other finite structures of the nanoscale size range. Synthesis, assembly, transport, reactivity, and stability of such structures are considered. Development of in-situ and ex-situ instrumentation for characterization of nanoparticles and their interfaces should be based on new principles for probing properties and phenomena not well understood at the nanometer scale. Modeling and simulation may include atom-based quantum mechanics; molecular dynamics; single-particle, multi-body and continuum based models; fractals; other methods suitable for modeling particle synthesis, assembling and interaction processes. Realization and application of systems, structures and devices with novel functions obtained via precursor nanoparticles is emphasized. Approaches may include gas-, liquid-, solid-, and vacuum-based processes, size reduction, chemical- and bio-self assembly. Contributions include utilization of nanoparticle systems for enhancing a phenomenon or process and particle assembling into hierarchical structures, as well as formulation and the administration of drugs. Synergistic approaches originating from different disciplines and technologies, and interaction between the research providers and users in this field, are encouraged.