Noninvasive in-vivo human split-faced clinical investigation of nonanedioic acid loaded mesoporous silica nanoparticles immersed gel: revolutionizing cutaneous translational and cosmetical applications

Abstract

This study assesses the effectiveness of nonanedioic acid (NAA)-loaded mesoporous silica nanoparticles (MSNPs) in a 2% Carbopol gel for cutaneous restoration and anti-melanogenic therapy. The gel’s potential was evaluated through characterization and non-invasive skin hydration, elasticity, pigmentation, and rejuvenation tests. To verify amorphousness, thermal stability, and chemical makeup, MSNPs and NAA-MSNPs were characterized. The antioxidant activity of NAA and NAA-MSNPs was measured using the DPPH method, and spreadability tests were performed. The effects of the gel on the levels of melanin and erythema, sebum production, skin elasticity, moisture content, and facial pores were assessed using noninvasive in vivo tests. Patch tests on human volunteers demonstrated no skin irritation, and a panel test estimated the gel’s seven sensory and functional parameters. NAA-MSNPs showed improved antibacterial and antifungal activity compared to free NAA. SEM images showed spherical MSNPs and trials with human volunteers demonstrated significant improvements in skin parameters, including erythema, melanin index, hydration level, elasticity, sebum production, and facial pores, as illustrated by 3D visioFace imaging. Notably, the NAA-MSNP gel significantly reduced melanin levels by 16% after 12 weeks, enhancing skin hydration and elasticity. These results suggest that the NAA-MSNP-infused gel is a promising carrier for enhancing the efficacy of nonanedioic acid in skin care translational and cosmetical applications, particularly for depigmentation and anti-aging effects.

Graphical abstract