Photoactivated antimicrobial activity of carbon dots in combination with different co-agents and mechanistic implications

IF 2.6 4区材料科学 Q3 CHEMISTRY, MULTIDISCIPLINARY

Journal of Nanoparticle Research Pub Date : 2025-06-14 DOI:10.1007/s11051-025-06364-5

Cristian E. Rodriguez, Audrey F. Adcock, Xiuli Dong, Subhadra Yerra, Ya-Ping Sun, Liju Yang

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引用次数: 0

Abstract

Combating antibiotic-resistant bacteria has been a long-lasting challenge. This study investigated the photoactivated antibacterial function of carbon dots (CDots) in combination with antibiotics and membrane destabilizing agents as co-agents against drug resistant bacteria E. faecalis and L. monocytogenes. The results of the study demonstrated that CDots in combination with the membrane destabilizing agent poly-L-lysine as co-agent exhibited significant enhancement in the overall antibacterial activity, especially with the longer poly-L-lysine, achieving complete inactivation of cells in the samples with ~ 8 log viable cell reduction. The combination of CDots with bacterial resistant antibiotics streptomycin, erythromycin, or tetracycline, did not expectedly enhance the overall antibacterial activity of photoactive CDots to E. faecalis, in contrast, the combination with streptomycin significantly reduced the antibacterial activity of CDots. Implications of these experimental results toward the understanding of mechanistical action of photoexcited CDots were discussed. The study demonstrated that appropriately selected co-agents could effectively enhance the photoactivated antibacterial function of CDots and its potential to combat drug resistant pathogens.

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碳点与不同助剂组合的光激活抗菌活性及其机制意义

对抗耐抗生素细菌一直是一个长期的挑战。本实验研究了碳点（CDots）与抗生素和膜不稳定剂联合对耐药细菌粪肠杆菌和单核增生乳杆菌的光激活抗菌作用。研究结果表明，CDots与膜不稳定剂聚l -赖氨酸联合使用时，整体抗菌活性显著增强，特别是与较长的聚l -赖氨酸联合使用，可使样品中的细胞完全失活，活细胞减少~ 8 log。CDots与细菌耐药抗生素链霉素、红霉素或四环素联合使用，并没有增强光活性CDots对粪肠杆菌的整体抗菌活性，相反，与链霉素联合使用显著降低了CDots的抗菌活性。讨论了这些实验结果对理解光激发CDots的力学作用的意义。研究表明，选择合适的协同剂可以有效增强CDots的光激活抗菌功能及其对抗耐药病原体的潜力。

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来源期刊

Journal of Nanoparticle Research 工程技术-材料科学：综合

CiteScore

4.40

自引率

4.00%

发文量

198

审稿时长

3.9 months

期刊介绍： The objective of the Journal of Nanoparticle Research is to disseminate knowledge of the physical, chemical and biological phenomena and processes in structures that have at least one lengthscale ranging from molecular to approximately 100 nm (or submicron in some situations), and exhibit improved and novel properties that are a direct result of their small size. Nanoparticle research is a key component of nanoscience, nanoengineering and nanotechnology. The focus of the Journal is on the specific concepts, properties, phenomena, and processes related to particles, tubes, layers, macromolecules, clusters and other finite structures of the nanoscale size range. Synthesis, assembly, transport, reactivity, and stability of such structures are considered. Development of in-situ and ex-situ instrumentation for characterization of nanoparticles and their interfaces should be based on new principles for probing properties and phenomena not well understood at the nanometer scale. Modeling and simulation may include atom-based quantum mechanics; molecular dynamics; single-particle, multi-body and continuum based models; fractals; other methods suitable for modeling particle synthesis, assembling and interaction processes. Realization and application of systems, structures and devices with novel functions obtained via precursor nanoparticles is emphasized. Approaches may include gas-, liquid-, solid-, and vacuum-based processes, size reduction, chemical- and bio-self assembly. Contributions include utilization of nanoparticle systems for enhancing a phenomenon or process and particle assembling into hierarchical structures, as well as formulation and the administration of drugs. Synergistic approaches originating from different disciplines and technologies, and interaction between the research providers and users in this field, are encouraged.