Integrated Microfluidic Extraction and Detection of Aflatoxin B1 in Olive Oil

IF 3 3区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Food Analytical Methods Pub Date : 2025-06-14 DOI:10.1007/s12161-025-02827-6

Nagihan Okutan Arslan, Levent Trabzon

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引用次数: 0

Abstract

Food microfluidics are powerful tools to create total analysis systems and have long been demonstrated to be useful for safety and quality applications. This study introduced a new integrated aflatoxin B1 extraction and detection system based on microfluidic technology. A poly(dimethylsiloxane) microfluidic mixer was developed herein to rapidly extract aflatoxin B1 from olive oil samples. We believe that the sunflower microfluidic mixer developed in this study can be widely applied in a variety of related fields that are extremely difficult in traditional batch processes. Successful integration of microfluidic mixers brings us closer to one-step detection. The used approach allows for analysis with only 2 ml of sample. The disposable and cost-effective paper-based microfluidics were utilized to construct the immunosensor for aflatoxin B1 detection. Owing to the high surface area of paper and carbon nanotubes sensitive measurement can be done at le 0.01 nanogram levels, below the regulatory requirements. Further, the paper-based microfluidic exhibited high recoveries between 91 and 97% for aflatoxin B1 detection in olive oil, demonstrating the method’s potential for use in the study of a variety of agricultural and culinary goods.

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橄榄油中黄曲霉毒素B1的集成微流控提取与检测

食品微流体是创建整体分析系统的强大工具，长期以来一直被证明对安全和质量应用是有用的。介绍了一种基于微流控技术的新型黄曲霉毒素B1综合提取检测系统。本文研制了一种聚二甲基硅氧烷微流控混合器，用于快速提取橄榄油样品中的黄曲霉毒素B1。我们相信，本研究开发的向日葵微流控混合器可以广泛应用于传统间歇式工艺中极为困难的各种相关领域。微流控混合器的成功集成使我们更接近一步检测。所使用的方法允许只用2毫升的样品进行分析。利用一次性、高性价比的纸基微流体构建黄曲霉毒素B1免疫传感器。由于纸张和碳纳米管的高表面积，可以在0.01纳克的水平下进行敏感测量，低于监管要求。此外，基于纸张的微流体检测橄榄油中黄曲霉毒素B1的回收率在91%到97%之间，表明该方法在各种农业和烹饪产品的研究中具有潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Food Analytical Methods 农林科学-食品科技

CiteScore

6.00

自引率

3.40%

发文量

244

审稿时长

3.1 months

期刊介绍： Food Analytical Methods publishes original articles, review articles, and notes on novel and/or state-of-the-art analytical methods or issues to be solved, as well as significant improvements or interesting applications to existing methods. These include analytical technology and methodology for food microbial contaminants, food chemistry and toxicology, food quality, food authenticity and food traceability. The journal covers fundamental and specific aspects of the development, optimization, and practical implementation in routine laboratories, and validation of food analytical methods for the monitoring of food safety and quality.