Surface-Enhanced Raman Spectroscopy for Characterization of Blood Serum Samples of Liver Carcinoma Patients Using 100 kDa Filtration Devices

IF 4.3 4区物理与天体物理 Q2 CHEMISTRY, PHYSICAL

Plasmonics Pub Date : 2025-04-10 DOI:10.1007/s11468-025-02941-5

Saima Afzal, Seher Nawabzadi, Haq Nawaz, Muhammad Irfan Majeed, Hanen Karamti, Muhammad Hafeez Ullah, Habiba Shahid, Muntaha Saleem, Rabia Tariq, Ayesha Saif, Sana Mehmood, Aneela Falak Sher, Rafia Atta, Munazza Hakeem, Muhammad Imran

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引用次数: 0

Abstract

Early detection of liver carcinoma is considered very important to overcome the growing mortalities worldwide. Surface-enhanced Raman spectroscopy (SERS) is a highly potential diagnostic technique for accurate analysis of 100 kDa filtrate portions of liver carcinoma patients. In this research work, blood serum samples were analyzed which contain low molecular weight fraction (LMWF) and high molecular weight fraction (HMWF) of biomolecules but later suppressed the earlier which are considered as disease biomarkers. However, the ultra-centrifugal devices having cut off values of 100 kDa are found to be useful to separate biomolecules on the basis of their molecular weight in the form of filtrate, with molecular weight less than 100 KDa and residue with molecular weight greater than 100 KDa. The chemometric tools including principal component analysis (PCA) and partial least square discriminant analysis (PLS-DA) were applied for the classification and differentiation of spectral data of filtrate samples of healthy and different stages of liver carcinoma. The significant SERS bands appeared at 655, 908, 955, 1127, 1250, and 1323 cm⁻¹ are directly associated with the progression of liver carcinoma disease.

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使用100 kDa过滤装置的表面增强拉曼光谱用于肝癌患者血清样品的表征

肝癌的早期发现被认为对克服世界范围内不断增长的死亡率非常重要。表面增强拉曼光谱（SERS）是一种非常有潜力的诊断技术，可以准确分析肝癌患者100 kDa的滤液部分。在本研究中，分析了血清样品中含有低分子量部分（LMWF）和高分子量部分（HMWF）的生物分子，这些生物分子被认为是疾病的生物标志物，但前者后来被抑制。然而，发现截留值为100 kDa的超离心装置可用于以滤液形式分离分子量小于100 kDa的生物分子和分子量大于100 kDa的残留物。应用主成分分析（PCA）和偏最小二乘判别分析（PLS-DA）等化学计量学工具对健康肝癌和不同分期肝癌滤液样品的光谱数据进行分类和鉴别。在655、908、955、1127、1250和1323 cm−1处出现显著的SERS谱带，与肝癌的进展直接相关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plasmonics 工程技术-材料科学：综合

CiteScore

5.90

自引率

6.70%

发文量

164

审稿时长

2.1 months

期刊介绍： Plasmonics is an international forum for the publication of peer-reviewed leading-edge original articles that both advance and report our knowledge base and practice of the interactions of free-metal electrons, Plasmons. Topics covered include notable advances in the theory, Physics, and applications of surface plasmons in metals, to the rapidly emerging areas of nanotechnology, biophotonics, sensing, biochemistry and medicine. Topics, including the theory, synthesis and optical properties of noble metal nanostructures, patterned surfaces or materials, continuous or grated surfaces, devices, or wires for their multifarious applications are particularly welcome. Typical applications might include but are not limited to, surface enhanced spectroscopic properties, such as Raman scattering or fluorescence, as well developments in techniques such as surface plasmon resonance and near-field scanning optical microscopy.