Nano-magnetic sorted CD29 +/CD44 +/CD105 + Bone MSCs alleviate cyclosporine nephrotoxicity and renal fibrosis

IF 2.6 4区材料科学 Q3 CHEMISTRY, MULTIDISCIPLINARY

Journal of Nanoparticle Research Pub Date : 2025-07-11 DOI:10.1007/s11051-025-06388-x

Pingbao Zhang, Cuidi Xu, Xinhao Niu, Yongshen Luo, Jingjing Guo, Xiaoqing Xu, Hao Zen, Lifei Liang, Xiaotian Yan, Jiahen Wu, Ruiming Rong

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引用次数: 0

Abstract

CD29/CD44/CD105-modified immunomagnetic liposomes (CD29/CD44/CD105-IMLs) were developed to isolate bone marrow mesenchymal stem cells (BMSCs) with distinct phenotypes. A key BMSC phenotype capable of alleviating chronic cyclosporine-induced nephropathy was identified through in vivo experiments in mice, demonstrating its potential as an effective adjunctive therapy for mitigating renal fibrosis-induced injury. Four weeks post-transplantation, mice receiving CD29⁺, CD44⁺, and CD105⁺ BMSC groups exhibited improved overall health compared to the control group. Western blot analysis of autophagy-related proteins LC3-II and P62 revealed significantly lower expression levels in the CD44⁺ BMSC group compared to the CD29⁺ and CD105⁺ BMSC groups. In vivo imaging further demonstrated enhanced chemotactic ability of CD44⁺ BMSCs in mice with chronic cyclosporine-induced nephropathy at various time points. These findings suggest that transplantation of CD29⁺/CD44⁺/CD105⁺ BMSCs can delay the progression of renal fibrosis in mice with cyclosporine-induced chronic kidney disease. Notably, CD44⁺ BMSCs exhibited superior efficacy in alleviating renal fibrosis compared to CD29⁺ and CD105⁺ BMSC groups.

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纳米磁分选CD29 +/CD44 +/CD105 +骨间充质干细胞减轻环孢素肾毒性和肾纤维化

CD29/CD44/ cd105修饰的免疫磁性脂质体（CD29/CD44/CD105-IMLs）用于分离具有不同表型的骨髓间充质干细胞（BMSCs）。通过小鼠体内实验确定了一种能够减轻慢性环孢素诱导肾病的关键BMSC表型，证明了其作为减轻肾纤维化诱导损伤的有效辅助疗法的潜力。移植后四周，与对照组相比，接受CD29+、CD44+和CD105+ BMSC治疗的小鼠整体健康状况有所改善。自噬相关蛋白LC3-II和P62的Western blot分析显示，与CD29+和CD105+ BMSC组相比，CD44+ BMSC组的表达水平显著降低。体内成像进一步显示，CD44+骨髓间充质干细胞在不同时间点的慢性环孢素肾病小鼠的趋化能力增强。这些发现表明，CD29+/CD44+/CD105+ BMSCs移植可以延缓环孢素诱导的慢性肾病小鼠肾纤维化的进展。值得注意的是，与CD29+和CD105+ BMSC组相比，CD44+ BMSC在减轻肾纤维化方面表现出更优越的疗效。

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来源期刊

Journal of Nanoparticle Research 工程技术-材料科学：综合

CiteScore

4.40

自引率

4.00%

发文量

198

审稿时长

3.9 months

期刊介绍： The objective of the Journal of Nanoparticle Research is to disseminate knowledge of the physical, chemical and biological phenomena and processes in structures that have at least one lengthscale ranging from molecular to approximately 100 nm (or submicron in some situations), and exhibit improved and novel properties that are a direct result of their small size. Nanoparticle research is a key component of nanoscience, nanoengineering and nanotechnology. The focus of the Journal is on the specific concepts, properties, phenomena, and processes related to particles, tubes, layers, macromolecules, clusters and other finite structures of the nanoscale size range. Synthesis, assembly, transport, reactivity, and stability of such structures are considered. Development of in-situ and ex-situ instrumentation for characterization of nanoparticles and their interfaces should be based on new principles for probing properties and phenomena not well understood at the nanometer scale. Modeling and simulation may include atom-based quantum mechanics; molecular dynamics; single-particle, multi-body and continuum based models; fractals; other methods suitable for modeling particle synthesis, assembling and interaction processes. Realization and application of systems, structures and devices with novel functions obtained via precursor nanoparticles is emphasized. Approaches may include gas-, liquid-, solid-, and vacuum-based processes, size reduction, chemical- and bio-self assembly. Contributions include utilization of nanoparticle systems for enhancing a phenomenon or process and particle assembling into hierarchical structures, as well as formulation and the administration of drugs. Synergistic approaches originating from different disciplines and technologies, and interaction between the research providers and users in this field, are encouraged.