Ultrasound-assisted biosynthesis of gelatin-gold nanocomposite: investigation of its antioxidant activity and its performance as anti-endometrial cancer in vitro

IF 1.7 4区化学 Q3 CHEMISTRY, INORGANIC & NUCLEAR

Transition Metal Chemistry Pub Date : 2025-03-10 DOI:10.1007/s11243-025-00640-y

Ying Shao, Na Hu, Jun Zhang, Attalla F. El-kott, Sally Negm

{"title":"Ultrasound-assisted biosynthesis of gelatin-gold nanocomposite: investigation of its antioxidant activity and its performance as anti-endometrial cancer in vitro","authors":"Ying Shao, Na Hu, Jun Zhang, Attalla F. El-kott, Sally Negm","doi":"10.1007/s11243-025-00640-y","DOIUrl":null,"url":null,"abstract":"<div><p>The current study outlines the simple process of designing Au nanoparticles (NPs) under mild conditions using gelatin as a natural capping and reducing agent. Through the use of UV–Vis (ultraviolet–visible), FT-IR (Fourier transform infrared) spectroscopy, TEM (transmission electron microscopy), FE-SEM (field emission scanning electron microscopy), EDX (energy-dispersive X-ray spectroscopy), XRD (X-ray diffraction), and elemental mapping techniques, the formation of gold nanoparticles stabilized by gelatin (Au NPs@Gel) and their structural properties were investigated. Gelatin-capped Au NPs produced the spherical, lowest average particle sizes, ranging from 10 to 15 nm, according to the microscopic images. Additionally, Au NPs/Gelatin showed a notably high percentage of cytotoxicity when tested against common human endometrial cell lines, such as AN3-CA and HEC-1-A, but a very low percentage of toxicity when tested against normal cell lines. Au NPs@Gel showed the strongest anti-endometrial effects against the AN3-CA cell line. The DPPH (2,2-Diphenyl-1-picrylhydrazyl) experiment was employed to examine the antioxidant characteristics of the Au NPs@Gel bio-composite, with BHT (butylated hydroxytoluene) serving as the positive control. IC<sub>50</sub> of the Au NPs@Gel material was found to be 213.8 µg/mL in the DPPH assay. Antioxidant activity of the Au NPs@Gel was strongly correlated with its capacity to prevent endometrial cancer. According to the aforementioned results, Au NPs@Gel may be used to treat a variety of human cancers.</p></div>","PeriodicalId":803,"journal":{"name":"Transition Metal Chemistry","volume":"50 4","pages":"555 - 566"},"PeriodicalIF":1.7000,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Transition Metal Chemistry","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s11243-025-00640-y","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, INORGANIC & NUCLEAR","Score":null,"Total":0}

引用次数: 0

Abstract

The current study outlines the simple process of designing Au nanoparticles (NPs) under mild conditions using gelatin as a natural capping and reducing agent. Through the use of UV–Vis (ultraviolet–visible), FT-IR (Fourier transform infrared) spectroscopy, TEM (transmission electron microscopy), FE-SEM (field emission scanning electron microscopy), EDX (energy-dispersive X-ray spectroscopy), XRD (X-ray diffraction), and elemental mapping techniques, the formation of gold nanoparticles stabilized by gelatin (Au NPs@Gel) and their structural properties were investigated. Gelatin-capped Au NPs produced the spherical, lowest average particle sizes, ranging from 10 to 15 nm, according to the microscopic images. Additionally, Au NPs/Gelatin showed a notably high percentage of cytotoxicity when tested against common human endometrial cell lines, such as AN3-CA and HEC-1-A, but a very low percentage of toxicity when tested against normal cell lines. Au NPs@Gel showed the strongest anti-endometrial effects against the AN3-CA cell line. The DPPH (2,2-Diphenyl-1-picrylhydrazyl) experiment was employed to examine the antioxidant characteristics of the Au NPs@Gel bio-composite, with BHT (butylated hydroxytoluene) serving as the positive control. IC₅₀ of the Au NPs@Gel material was found to be 213.8 µg/mL in the DPPH assay. Antioxidant activity of the Au NPs@Gel was strongly correlated with its capacity to prevent endometrial cancer. According to the aforementioned results, Au NPs@Gel may be used to treat a variety of human cancers.

Abstract Image

查看原文本刊更多论文

超声辅助生物合成明胶-金纳米复合物：体外抗氧化活性及抗子宫内膜癌性能的研究

目前的研究概述了在温和条件下使用明胶作为天然封盖和还原剂设计金纳米颗粒（NPs）的简单过程。利用UV-Vis（紫外-可见）、FT-IR（傅里叶变换红外）光谱、TEM（透射电子显微镜）、FE-SEM（场发射扫描电子显微镜）、EDX（能量色散x射线光谱）、XRD （x射线衍射）和元素作图技术，研究了明胶稳定金纳米颗粒（Au NPs@Gel）的形成及其结构性质。根据显微图像，明胶覆盖的Au NPs产生球形，平均粒径最低，范围从10到15纳米。此外，Au NPs/明胶在对常见的人子宫内膜细胞系（如AN3-CA和HEC-1-A）进行测试时显示出很高的细胞毒性百分比，但在对正常细胞系进行测试时毒性百分比非常低。Au NPs@Gel对AN3-CA细胞系的抗子宫内膜作用最强。采用DPPH（2,2-二苯基-1-picrylhydrazyl）实验，以BHT （butylated hydroxytoluene）为阳性对照，考察Au NPs@Gel生物复合材料的抗氧化性能。DPPH检测发现Au NPs@Gel材料的IC50为213.8µg/mL。Au NPs@Gel的抗氧化活性与其预防子宫内膜癌的能力密切相关。根据上述结果，Au NPs@Gel可用于治疗多种人类癌症。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Transition Metal Chemistry 化学-无机化学与核化学

CiteScore

3.60

自引率

0.00%

发文量

审稿时长

1.3 months

期刊介绍： Transition Metal Chemistry is an international journal designed to deal with all aspects of the subject embodied in the title: the preparation of transition metal-based molecular compounds of all kinds (including complexes of the Group 12 elements), their structural, physical, kinetic, catalytic and biological properties, their use in chemical synthesis as well as their application in the widest context, their role in naturally occurring systems etc. Manuscripts submitted to the journal should be of broad appeal to the readership and for this reason, papers which are confined to more specialised studies such as the measurement of solution phase equilibria or thermal decomposition studies, or papers which include extensive material on f-block elements, or papers dealing with non-molecular materials, will not normally be considered for publication. Work describing new ligands or coordination geometries must provide sufficient evidence for the confident assignment of structural formulae; this will usually take the form of one or more X-ray crystal structures.