Cytotoxic Effects of Particulate Matter on Cell Growth and Metabolism of Green Fluorescent Protein-Expressing Escherichia coli

IF 3.2 4区 工程技术 Q2 CHEMISTRY, MULTIDISCIPLINARY
Young Hoon Song, Hye Ryoung Heo, Ae Sol Lee, Chang Sup Kim, Jeong Hyun Seo
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Abstract

The toxicity of sulfate (SO4−2) and ammonium (NH4+), key components of fine dust, on living organisms was investigated using recombinant green fluorescent protein (GFP)-expressing Escherichia coli as a bioindicator. The effects of individual and mixed particulate matter (PM) compounds, including CuSO₄, (NH₄)₂SO₄, and NH₄Cl, were evaluated by measuring the optical density and GFP fluorescence intensity. Escherichia coli growth was inhibited by the individual compounds at specific thresholds, with CuSO₄ being most toxic at as low as 3.8 mM. Synergistic effects were observed with mixed compounds, markedly reducing growth and fluorescence even at lower concentrations. Notably, a mixture of the three at their sub-lethal individual concentrations completely halted bacterial growth after 2 h of incubation. CuSO₄ was a more potent inhibitor than (NH₄)₂SO₄ and NH₄Cl. These findings highlighted the importance of analyzing the individual and synergistic effects of PM components.

颗粒物质对表达绿色荧光蛋白的大肠杆菌细胞生长和代谢的细胞毒作用
以表达重组绿色荧光蛋白(GFP)的大肠杆菌为生物指示剂,研究了细颗粒物的关键成分硫酸盐(SO4−2)和铵(NH4+)对生物的毒性。通过测量光密度和GFP荧光强度,评价了单个和混合颗粒物质(PM)化合物CuSO₄、(NH₄)₂SO₄和NH₄Cl的影响。在特定阈值下,单个化合物对大肠杆菌的生长有抑制作用,其中硫酸铜在3.8 mM时毒性最大。混合化合物具有协同效应,即使在较低浓度下也能显著降低生长和荧光。值得注意的是,这三种物质的亚致死浓度混合物在孵育2小时后完全阻止了细菌的生长。CuSO₄是比(NH₄)2so₄和NH₄Cl更有效的抑制剂。这些发现强调了分析PM成分的个体和协同效应的重要性。
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来源期刊
Korean Journal of Chemical Engineering
Korean Journal of Chemical Engineering 工程技术-工程:化工
CiteScore
4.60
自引率
11.10%
发文量
310
审稿时长
4.7 months
期刊介绍: The Korean Journal of Chemical Engineering provides a global forum for the dissemination of research in chemical engineering. The Journal publishes significant research results obtained in the Asia-Pacific region, and simultaneously introduces recent technical progress made in other areas of the world to this region. Submitted research papers must be of potential industrial significance and specifically concerned with chemical engineering. The editors will give preference to papers having a clearly stated practical scope and applicability in the areas of chemical engineering, and to those where new theoretical concepts are supported by new experimental details. The Journal also regularly publishes featured reviews on emerging and industrially important subjects of chemical engineering as well as selected papers presented at international conferences on the subjects.
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