A Multifunctional Pectinase from the Manglicolous Endophytic Fungus, Aspergillus sydowii EF 375: Process Optimization, Characterization and Application Potentials
M R Revathy, Anjali S. Mohan, Dhanya Kesavan, Hafsa Kattatheyil, S Neelima, M V Anju, Rosamma Philip
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Abstract
Microbial pectinases, classified within the hydrolase family, play a crucial role in various industrial applications, including food and beverage processing, textile manufacturing, and wastewater treatment. This study investigates the production, purification, biochemical characterization, and potential applications of pectinase derived from the endophytic fungal strain Aspergillus sydowii EF 375, isolated from the mangrove plant Ceriops tagal. Pectinase production was optimized using Response Surface Methodology (RSM), resulting in a 2.2-fold enhancement in enzyme production. The enzyme was subsequently purified, achieving a recovery yield of 3.2% and a specific activity of 73.05 U/mg. Zymogram analysis confirmed the purified pectinase to have a molecular weight of approximately 43 kDa. Kinetic studies revealed a maximum reaction velocity (Vmax) of 161.29 µmol/min and a Michaelis constant (Km) of 0.177 mg/ml, indicating high substrate affinity. The enzyme exhibited optimal catalytic efficiency at 50 °C and pH 5. The activity of the enzyme was notably enhanced by the presence of K⁺, Cu2⁺, Fe2⁺, Mg2⁺, Mn2⁺, Pb2⁺, and Zn2⁺ ions (at concentrations up to 10 mM), while Li⁺, Hg2⁺, and Ni2⁺ acted as inhibitors. Functional applications demonstrated that the purified pectinase significantly improved the clarity of fruit juices, including apple, grape, pineapple, and orange. Furthermore, the enzyme exhibited potential in inhibiting bacterial biofilm formation, highlighting its applicability in antimicrobial and biotechnological interventions.
期刊介绍:
Catalysis Letters aim is the rapid publication of outstanding and high-impact original research articles in catalysis. The scope of the journal covers a broad range of topics in all fields of both applied and theoretical catalysis, including heterogeneous, homogeneous and biocatalysis.
The high-quality original research articles published in Catalysis Letters are subject to rigorous peer review. Accepted papers are published online first and subsequently in print issues. All contributions must include a graphical abstract. Manuscripts should be written in English and the responsibility lies with the authors to ensure that they are grammatically and linguistically correct. Authors for whom English is not the working language are encouraged to consider using a professional language-editing service before submitting their manuscripts.