{"title":"Gas Chromatography-Mass Spectrometry-Based Metabolomics to Understand Metabolic Perturbations in Drosophila Due to Lead Acetate Exposure","authors":"Hussain Shaik, Mohana Krishna Reddy Mudiam","doi":"10.1134/S106193482570042X","DOIUrl":null,"url":null,"abstract":"<p>Lead acetate is recognized as one of the most toxic compounds, with the potential to adversely affect human health, the environment, and ecosystems. In this study, metabolic changes in <i>Drosophila</i> due to lead acetate exposure were analyzed using gas chromatography-mass spectrometry (<b>GC-MS</b>)-based metabolomics at sub-lethal concentrations (2.0 and 20 µg/mL). The study identified a total of 79 metabolites, of which eight were found to be significantly altered (hexadecenoic acid, propanoic acid, glucose, gluconic acid, alanine, melibiose, galactose, and isoleucine) in <i>Drosophila</i> due to lead acetate exposure. These were identified after multivariate analysis of the GC-MS metabolomics data with variable importance in projection scores greater than one. The significantly altered metabolic pathway in <i>Drosophila</i> was identified as aminoacyl-tRNA biosynthesis with an impact value of 0.069.</p>","PeriodicalId":606,"journal":{"name":"Journal of Analytical Chemistry","volume":"80 6","pages":"1077 - 1088"},"PeriodicalIF":1.1000,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Analytical Chemistry","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1134/S106193482570042X","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
Lead acetate is recognized as one of the most toxic compounds, with the potential to adversely affect human health, the environment, and ecosystems. In this study, metabolic changes in Drosophila due to lead acetate exposure were analyzed using gas chromatography-mass spectrometry (GC-MS)-based metabolomics at sub-lethal concentrations (2.0 and 20 µg/mL). The study identified a total of 79 metabolites, of which eight were found to be significantly altered (hexadecenoic acid, propanoic acid, glucose, gluconic acid, alanine, melibiose, galactose, and isoleucine) in Drosophila due to lead acetate exposure. These were identified after multivariate analysis of the GC-MS metabolomics data with variable importance in projection scores greater than one. The significantly altered metabolic pathway in Drosophila was identified as aminoacyl-tRNA biosynthesis with an impact value of 0.069.
期刊介绍：
The Journal of Analytical Chemistry is an international peer reviewed journal that covers theoretical and applied aspects of analytical chemistry; it informs the reader about new achievements in analytical methods, instruments and reagents. Ample space is devoted to problems arising in the analysis of vital media such as water and air. Consideration is given to the detection and determination of metal ions, anions, and various organic substances. The journal welcomes manuscripts from all countries in the English or Russian language.
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