Cardioprotective potential of azaleatin against sodium arsenite instigated sub-chronic cardiotoxicity via targeting TLR4/MyD88, JAK1/STAT3, and NF-κB in Sprague Dawley rats

IF 3.6 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Hesham M. Hassan , Aqsa Bibi , Muhammad Faisal Hayat , Khalid J. Alzahrani , Fuad M. Alzahrani , Meshari A. Alsuwat , Maha A. Al-Aream
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引用次数: 0

Abstract

Background

Sodium arsenite (SA) is a potent carcinogenic compound which is evident to prompt multiple organs damage including the heart. Azaleatin (AZA) is a novel therapeutic agent with excellent biological properties.

Objectives

The current investigation was executed to evaluate the mitigative efficacy of AZA against SA induced sub-chronic cardiotoxicity in rats through the evaluation of biochemical and histological parameters.

Methodology

Thirty-two rats were apportioned into control, SA (10 mg kg−1), SA (10 mg kg−1) + AZA (25 mg kg−1), and AZA (25 mg kg−1) exposed group. Biochemical parameters were evaluated through different techniques including qRT-PCR, ELISA and already reported standard assays. Histological analysis was performed to validate the tissue damage. The curative potential of AZA was re-validated through in-silico approaches.

Findings

SA intoxication upregulated the expression of nuclear factor-kappa B (NF-κB), myeloid differentiation primary response 88 (MyD88), janus kinase 1 (JAK1), toll-like receptor 4 (TLR4), tumor necrosis factor-α (TNF-α), signal transducer and activator of transcription 3 (STAT3), interleukin-1β (IL-1β), interleukin-6 (IL-6), & cyclooxygenase-2 (COX-2) while increasing the levels of reactive oxygen species (ROS) and malondialdehyde (MDA). Moreover, the enzymatic activities glutathione peroxidase (GPx), glutathione reductase (GSR), glutathione S-transferase (GST), superoxide dismutase (SOD), catalase (CAT), heme-oxygenase-1 (HO-1) and glutathione (GSH) were lowered whereas the levels of Troponin I, ProBNP, C-reactive protein, LDH, troponin-T, BNP and CPK were exacerbated following the SA exposure. Furthermore, SA intoxication promoted the levels of Bax, Caspase-9, and Caspase-3 while lowering the levels of Bcl-2. Cardiac tissues showed impaired histology after SA administration. Nonetheless, AZA therapy excellently ameliorated cardiac toxicity owing to its anti-inflammatory, antioxidative and anti-apoptotic potential.

Conclusion

SA intoxication induced severe cardiac toxicity via disrupting biochemical and histological parameters while AZA excellently restored cardiac health profile. Collectively, AZA may serve as a cardioprotective agent in counteracting SA induced sub-chronic cardiotoxicity.
azaleatin通过靶向TLR4/MyD88、JAK1/STAT3和NF-κB对亚砷酸钠诱导的Sprague Dawley大鼠亚慢性心脏毒性的心脏保护作用
背景:亚砷酸钠(SA)是一种强致癌物,可引起包括心脏在内的多个器官损伤。Azaleatin (AZA)是一种具有优良生物学特性的新型治疗剂。目的通过生化和组织学指标评价AZA对SA诱导的大鼠亚慢性心脏毒性的缓解作用。方法将32只大鼠分为对照组、SA (10 mg kg−1)、SA (10 mg kg−1)+ AZA (25 mg kg−1)和AZA (25 mg kg−1)暴露组。生化参数通过不同的技术进行评估,包括qRT-PCR, ELISA和已经报道的标准分析。组织学分析证实组织损伤。通过计算机方法重新验证了AZA的治疗潜力。结果发现,sa中毒可上调核因子-κB (NF-κB)、髓样分化初级反应88 (MyD88)、janus激酶1 (JAK1)、toll样受体4 (TLR4)、肿瘤坏死因子-α (TNF-α)、信号转导和转录激活因子3 (STAT3)、白细胞介素-1β (IL-1β)、白细胞介素-6 (IL-6)、环氧化酶-2 (COX-2)的表达,同时增加活性氧(ROS)和丙二醛(MDA)的水平。此外,SA暴露后,谷胱甘肽过氧化物酶(GPx)、谷胱甘肽还原酶(GSR)、谷胱甘肽s转移酶(GST)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、血红素加氧酶-1 (HO-1)和谷胱甘肽(GSH)活性降低,而肌钙蛋白I、ProBNP、c反应蛋白、LDH、肌钙蛋白-t、BNP和CPK水平升高。此外,SA中毒提高了Bax、Caspase-9和Caspase-3的水平,同时降低了Bcl-2的水平。给药后心脏组织出现组织学损伤。然而,由于其抗炎、抗氧化和抗凋亡的潜力,AZA治疗可以很好地改善心脏毒性。结论sa中毒通过破坏生物化学和组织学参数引起严重的心脏毒性,而AZA能很好地恢复心脏健康状况。总的来说,AZA可以作为一种心脏保护剂来对抗SA诱导的亚慢性心脏毒性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
6.60
自引率
2.90%
发文量
202
审稿时长
85 days
期刊介绍: The journal provides the reader with a thorough description of theoretical and applied aspects of trace elements in medicine and biology and is devoted to the advancement of scientific knowledge about trace elements and trace element species. Trace elements play essential roles in the maintenance of physiological processes. During the last decades there has been a great deal of scientific investigation about the function and binding of trace elements. The Journal of Trace Elements in Medicine and Biology focuses on the description and dissemination of scientific results concerning the role of trace elements with respect to their mode of action in health and disease and nutritional importance. Progress in the knowledge of the biological role of trace elements depends, however, on advances in trace elements chemistry. Thus the Journal of Trace Elements in Medicine and Biology will include only those papers that base their results on proven analytical methods. Also, we only publish those articles in which the quality assurance regarding the execution of experiments and achievement of results is guaranteed.
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