Molecular survey of hemotropic mycoplasmas and piroplasmids in cats (Felis catus) in Central Taiwan

IF 1.4 Q3 PARASITOLOGY

Veterinary parasitology, regional studies and reports Pub Date : 2025-09-21 DOI:10.1016/j.vprsr.2025.101348

Sijia Yu , Peihang Hong , Yu-Syuan Peng , Tzu-Yun Shih , Pin-Chen Liu , Chao-Min Wang , Cheng-Hung Lai , Shyun Chou

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Abstract

Data on feline hemotropic mycoplasmas and piroplasmids in Taiwan are limited. Therefore, the aim of this study was to investigate the prevalence of hemotropic mycoplasmas and piroplasmids in cats in central Taiwan, as well as to conduct a phylogenetic analysis of the detected pathogens. In total, blood samples of 213 cats were collected from three regions of central Taiwan: Taichung, Yunlin, and Nantou. Polymerase chain reaction (PCR) was used to amplify the 16S rRNA gene fragments of hemotropic mycoplasmas and the 18S rRNA gene fragments of piroplasmids. Risk factors were also assessed, and phylogenetic analysis was performed. Out of the 213 samples analyzed, the prevalence of hemotropic mycoplasmas was 10.80 % (23/213). Specifically, Candidatus Mycoplasma haemominutum, Mycoplasma haemofelis, and Mycoplasma sp. were detected in 7.51 % (16/213), 2.82 % (6/213), and 0.47 % (1/213) of the samples, respectively. Only Babesia gibsoni was detected among the piroplasmids, with a positivity rate of 0.47 % (1/213). No statistically significant associations were found between hemotropic mycoplasmas and the associated factors. Phylogenetic analysis suggested that the uncultured Mycoplasma sp. identified may represent a previously unidentified Mycoplasma species, potentially related to uncultured Mycoplasma spp. detected in cats from Iran. In conclusion, this study identified B. gibsoni in feline blood samples from Taiwan and provides evidence suggesting the existence of unidentified Mycoplasma species capable of infecting cats.

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台湾中部猫类嗜血血支原体及梨质粒之分子调查

台湾有关猫科嗜血血支原体和梨质粒的资料有限。因此，本研究的目的是调查台湾中部地区猫中嗜血血支原体和梨质粒的流行情况，并对检测到的病原体进行系统发育分析。在台中、云林、南投三个地区共采集了213只猫的血液样本。采用聚合酶链反应（PCR）对嗜血性支原体的16S rRNA基因片段和螺质粒的18S rRNA基因片段进行扩增。同时对危险因素进行评估，并进行系统发育分析。在分析的213份样本中，嗜血性支原体的患病率为10.80%（23/213）。其中，血支原体、嗜血支原体和支原体分别占7.51%（16/213）、2.82%（6/213）和0.47%（1/213）。piro质粒中仅检出巴贝斯虫，阳性率为0.47%（1/213）。嗜血性支原体与相关因素之间无统计学意义的关联。系统发育分析表明，鉴定的未培养支原体可能代表以前未识别的支原体物种，可能与在伊朗猫中检测到的未培养支原体有关。综上所述，本研究在台湾的猫血样本中鉴定出了gibsoni支原体，并提供了能够感染猫的未知支原体种类存在的证据。

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来源期刊

Veterinary parasitology, regional studies and reports Multiple-

CiteScore

2.90

自引率

7.10%

发文量

126

审稿时长

97 days

期刊介绍： Veterinary Parasitology: Regional Studies and Reports focuses on aspects of veterinary parasitology that are of regional concern, which is especially important in this era of climate change and the rapid and often unconstrained travel of people and animals. Relative to regions, this journal will accept papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites within the field of veterinary medicine. Also, case reports will be considered as they add to information related to local disease and its control; such papers must be concise and represent appropriate medical intervention. Papers on veterinary parasitology from wildlife species are acceptable, but only if they relate to the practice of veterinary medicine. Studies on vector-borne bacterial and viral agents are suitable, but only if the paper deals with vector transmission of these organisms to domesticated animals. Studies dealing with parasite control by means of natural products, both in vivo and in vitro, are more suited for one of the many journals that now specialize in papers of this type. However, due to the regional nature of much of this research, submissions may be considered based upon a case being made by the author(s) to the Editor. Circumstances relating to animal experimentation must meet the International Guiding Principles for Biomedical Research Involving Animals as issued by the Council for International Organizations of Medical Sciences (obtainable from: Executive Secretary C.I.O.M.S., c/o W.H.O., Via Appia, CH-1211 Geneva 27, Switzerland).