Yuping Liu , Ning Zhang , Dier Song , Rui Liu , Yufan Nie , Yue Zhang , Wenwen Guan , Wentao Zhu , Zhiqiang Zhou , Jinling Diao
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引用次数: 0
Abstract
Chiral pesticide enantiomers often exhibit different bioactivities, ecotoxicity and environmental fate. In this study, the separation conditions were systematically optimized based on HPLC combined with chiral stationary phases. The novel chiral insecticide cyclaniliprole enantiomers were well separated using a CHIRALPAK AD-H column with hexane/isopropanol (80/20, v/v) as mobile phase at 1 mL/min flow rate and 25 °C column temperature. The absolute configuration of cyclaniliprole enantiomers was determined using density functional theory. Bioactivity and toxicity results showed S-CYP exhibited higher bioactivity against Myzus persica and Bemisia tabaci than R-CYP and racemate, while acute toxicity to Daphnia magna and Apis mellifera showed minimal enantioselectivity. The degradation half-life of R-CYP on strawberries (33.08d) was significantly higher than S-CYP (24.90d) and racemate (24.40d). Additionally, racemate and two enantiomers all promoted fruit growth and ripening by increasing GA, IAA, and ABA levels, with S-CYP showing more effective. However, fruit growth may come at the cost of sacrificing flavor. All three pesticide treatments triggered oxidative stress in strawberries, suppressed sugar-metabolism related genes expression, lowered soluble sugar content, and disrupted volatile synthesis, ultimately reducing fruit flavor. Moreover, cyclaniliprole and its enantiomers increased anthocyanins and vitamin C contents, but decreased total phenolic content. At the enantiomeric level, S-CYP suppressed volatiles more strongly but had less impact on sugars than R-CYP, and promoted nutrients more strongly. Therefore, from the perspective of strawberry fruit development and flavor quality, application of S-CYP was more superior. However, commercialization of the pure isomer requires further stereospecific data on its bioactivity, toxicity, and environmental behavior.
期刊介绍:
Pesticide Biochemistry and Physiology publishes original scientific articles pertaining to the mode of action of plant protection agents such as insecticides, fungicides, herbicides, and similar compounds, including nonlethal pest control agents, biosynthesis of pheromones, hormones, and plant resistance agents. Manuscripts may include a biochemical, physiological, or molecular study for an understanding of comparative toxicology or selective toxicity of both target and nontarget organisms. Particular interest will be given to studies on the molecular biology of pest control, toxicology, and pesticide resistance.
Research Areas Emphasized Include the Biochemistry and Physiology of:
• Comparative toxicity
• Mode of action
• Pathophysiology
• Plant growth regulators
• Resistance
• Other effects of pesticides on both parasites and hosts.