Gut microbiota alterations linked to classical scrapie in sheep

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science Pub Date : 2025-09-23 DOI:10.1016/j.rvsc.2025.105916

Adelaida Hernaiz , Laura Grasa , Paula De Diego Abejón , Celia León Huertas , Belén Marín , Juan José Badiola , Rosa Bolea , Pilar Zaragoza , Inmaculada Martín-Burriel

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引用次数: 0

Abstract

There is growing evidence of the involvement of gut microbiota in neurodegenerative diseases. However, the relationship between gut microbiota and prion diseases is not yet well understood. Prion diseases are neurodegenerative disorders affecting humans and animals and caused by an infectious misfolded protein known as prion. Among prion diseases, scrapie is the one affecting sheep and goats, and its classical form is typically acquired. In the present work, a 16S rRNA-based microbiome profiling was performed in faecal samples from 10 sheep infected with classical scrapie and 10 control sheep. The phyla Firmicutes and Bacteroidetes appeared as the most abundant phyla in scrapie and control sheep. Moreover, both groups of samples showed similar richness and alpha diversity indices. The gut microbiota profile in terms of beta diversity was significantly different in scrapie-infected sheep compared to the control group. Additionally, similarly to other neurodegenerative diseases, scrapie animals displayed a significant increased abundance of Cyanobacteria and Lentisphaerae phyla. Our results point to an altered gut microbiota in sheep infected with classical scrapie, with a specific increase of two phyla that could be involved in scrapie disease pathogenesis, enabling conceptual advances in the understanding of prion diseases.

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绵羊经典痒病的肠道菌群改变

越来越多的证据表明，肠道微生物群与神经退行性疾病有关。然而，肠道菌群与朊病毒疾病之间的关系尚不清楚。朊病毒疾病是一种影响人类和动物的神经退行性疾病，由一种被称为朊病毒的传染性错误折叠蛋白引起。在朊病毒疾病中，痒病是一种影响绵羊和山羊的疾病，其经典形式通常是获得性的。在本研究中，对10只感染经典痒病的绵羊和10只对照羊的粪便样本进行了基于16S rrna的微生物组分析。厚壁菌门和拟杆菌门是病羊和对照羊中数量最多的门。此外，两组样品的丰富度和α多样性指数相似。与对照组相比，瘙痒病感染羊的肠道微生物群特征在β多样性方面显着不同。此外，与其他神经退行性疾病相似，痒病动物显示蓝藻和慢藻门的丰度显著增加。我们的研究结果表明，感染经典痒病的绵羊肠道微生物群发生了改变，其中两个门的特异性增加可能涉及痒病的发病机制，从而使对朊病毒疾病的理解取得了概念上的进展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Research in veterinary science 农林科学-兽医学

CiteScore

4.40

自引率

4.20%

发文量

312

审稿时长

75 days

期刊介绍： Research in Veterinary Science is an International multi-disciplinary journal publishing original articles, reviews and short communications of a high scientific and ethical standard in all aspects of veterinary and biomedical research. The primary aim of the journal is to inform veterinary and biomedical scientists of significant advances in veterinary and related research through prompt publication and dissemination. Secondly, the journal aims to provide a general multi-disciplinary forum for discussion and debate of news and issues concerning veterinary science. Thirdly, to promote the dissemination of knowledge to a broader range of professions, globally. High quality papers on all species of animals are considered, particularly those considered to be of high scientific importance and originality, and with interdisciplinary interest. The journal encourages papers providing results that have clear implications for understanding disease pathogenesis and for the development of control measures or treatments, as well as those dealing with a comparative biomedical approach, which represents a substantial improvement to animal and human health. Studies without a robust scientific hypothesis or that are preliminary, or of weak originality, as well as negative results, are not appropriate for the journal. Furthermore, observational approaches, case studies or field reports lacking an advancement in general knowledge do not fall within the scope of the journal.