Transcriptomic evidence of Dicranopteris pedata’s specialized defense systems against REEs stress

IF 6.1 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Ecotoxicology and Environmental Safety Pub Date : 2025-09-27 DOI:10.1016/j.ecoenv.2025.119100

Wenzhi Zhou , Haiyan Wang , Yonghe Han , Yanglong Jiang , Zhibiao Chen

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引用次数: 0

Abstract

Dicranopteris pedata is a typical REEs-hyperaccumulator that efficiently takes up REEs from the soil. However, the molecule mechanisms of REEs absorption and transportation by D. pedata is still unclear. In this study, D. pedata was selected as the research subject and exposed it to varying concentrations of yttrium (Y) and cerium (Ce) to investigate its stress effects. The results revealed, low exogenous REEs concentration can promote the expression of photosynthesis genes, and higher REEs stress can impede this process. Moreover, the significant down-regulation of PstS, ABCA3, ABCB1, ABCC1, and ABCD3 of the ABC transporter family may reduce the adverse effects of REEs. D. pedata can alleviate oxidative damage by increasing the activity of SOD and CAT, but this mechanism fails under high REEs stress. In addition, D. pedata up-regulates the gene expression of flavonoids, brassinosteroids, lysine, isoquinoline alkaloids, and streptomycin biosynthesis to alleviate stress. Besides, D. pedata also up-regulated the expression of naphthalene, caprolactam, toluene, geraniol, and fluorobenzoate degradation genes, which may be related to the detoxification mechanism of D. pedata. This study elucidates the physiological and transcriptional regulatory mechanisms of REEs stress response in D. pedata, deepening our understanding of the response of D. pedata to REEs stress.

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脚爪蝶抗稀土胁迫的特殊防御系统的转录组学证据

凤尾蕨是典型的稀土超富集植物，能有效地吸收土壤中的稀土元素。然而，豆科植物吸收和运输稀土元素的分子机制尚不清楚。本研究选择D. pedata作为研究对象，将其暴露于不同浓度的钇(Y)和铈（Ce）中，研究其应激效应。结果表明，低外源稀土浓度可以促进光合基因的表达，而高外源稀土胁迫会阻碍这一过程。此外，ABC转运体家族的PstS、ABCA3、ABCB1、ABCC1和ABCD3的显著下调可能会减轻REEs的不良影响。牡丹可通过提高SOD和CAT的活性来减轻氧化损伤，但在高稀土胁迫下，这一机制失效。此外，D. peddata上调黄酮类、油菜素内酯、赖氨酸、异喹啉类生物碱和链霉素生物合成基因的表达以缓解应激。此外，D. pedata还上调了萘、己内酰胺、甲苯、香叶醇和氟苯甲酸酯降解基因的表达，这可能与D. pedata的解毒机制有关。本研究阐明了芥蓝对稀土胁迫响应的生理和转录调控机制，加深了我们对芥蓝对稀土胁迫响应的认识。

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来源期刊

Ecotoxicology and Environmental Safety 环境科学-毒理学

CiteScore

12.10

自引率

5.90%

发文量

1234

审稿时长

88 days

期刊介绍： Ecotoxicology and Environmental Safety is a multi-disciplinary journal that focuses on understanding the exposure and effects of environmental contamination on organisms including human health. The scope of the journal covers three main themes. The topics within these themes, indicated below, include (but are not limited to) the following: Ecotoxicology、Environmental Chemistry、Environmental Safety etc.