Expression levels of MMP-1, MMP-3, and TIMP-1 in pediatric tonsillar tissue: A comparative study of tonsillar hypertrophy and chronic tonsillitis

IF 1.3 4区医学 Q3 OTORHINOLARYNGOLOGY

International journal of pediatric otorhinolaryngology Pub Date : 2025-09-24 DOI:10.1016/j.ijporl.2025.112573

Seren Karabiber Cicek , Gul Acar , Ilknur Bingul , Serdal Celik

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引用次数: 0

Abstract

Objective

The aim of this study is to investigate the tissue levels of Matrix Metalloproteinase-1 (MMP-1), Matrix Metalloproteinase-3 (MMP-3), and Tissue Inhibitor of Matrix Metalloproteinase-1 (TIMP-1) in cases of chronic tonsillitis and tonsillar hypertrophy, in order to elucidate their mechanisms of action in inflammatory processes.

Materials and methods

A total of 72 pediatric patients with an indication for tonsillectomy were included in the study. Of these, 36 patients diagnosed with chronic tonsillitis comprised the chronic tonsillitis (CT)group, while 36 patients diagnosed with tonsillar hypertrophy formed the tonsillar hypertrophy (TH) group. Punch biopsies were obtained from the tonsillar tissue following tonsillectomy in all patients. The samples were homogenized, sonicated, and centrifuged, and the supernatant fluid was analyzed for MMP-1, MMP-3, and TIMP-1 tissue levels using ELISA protocols.

Results

In this study, MMP-1, MMP-3 and TIMP-1 levels and the rates of these biomarkers were examined in the chronic tonsillitis and hypertrophic tonsil groups, and no statistically significant difference was found between this two groups. Although a weak positive correlation was noted between age and MMP/TIMP values in both clinical groups, this was not statistically significant. Median values of MMP-1, MMP-3, and TIMP-1 appeared higher in females, but this difference was not statistically significant when compared to males.

Conclusion

In this study, tissue levels of MMP-1, MMP-3, and TIMP-1 and their ratios were evaluated in children diagnosed with chronic tonsillitis and tonsillar hypertrophy, with no significant differences detected between the groups. The findings suggest that the MMP/TIMP system maintains a stable balance in tonsillar tissue during childhood and does not appear to distinguish between chronic tonsillitis and tonsillar hypertrophy in clinical practice.

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MMP-1、MMP-3和TIMP-1在儿童扁桃体组织中的表达水平：扁桃体肥大与慢性扁桃体炎的比较研究

目的研究慢性扁桃体炎和扁桃体肥大患者组织中基质金属蛋白酶-1 （MMP-1）、基质金属蛋白酶-3 （MMP-3）和基质金属蛋白酶-1组织抑制剂（TIMP-1）的表达水平，探讨其在炎症过程中的作用机制。材料和方法本研究共纳入72例有扁桃体切除术指征的儿童患者。其中，36例诊断为慢性扁桃体炎的患者组成慢性扁桃体炎（CT）组，36例诊断为扁桃体肥大的患者组成扁桃体肥大（TH）组。所有患者扁桃体切除术后均行扁桃体组织穿刺活检。样品均质、超声和离心，上清液分析MMP-1、MMP-3和TIMP-1组织水平采用ELISA协议。结果本研究比较慢性扁桃体炎组和增生性扁桃体组中MMP-1、MMP-3、TIMP-1的表达水平及其表达率，两组间差异无统计学意义。尽管两组患者的年龄与MMP/TIMP值之间存在微弱的正相关，但无统计学意义。MMP-1、MMP-3和TIMP-1的中位数在女性中较高，但与男性相比差异无统计学意义。结论本研究检测慢性扁桃体炎和扁桃体肥大患儿组织中MMP-1、MMP-3和TIMP-1的水平及其比值，各组间差异无统计学意义。研究结果表明，MMP/TIMP系统在儿童时期扁桃体组织中保持稳定的平衡，并且在临床实践中似乎无法区分慢性扁桃体炎和扁桃体肥大。

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来源期刊

International journal of pediatric otorhinolaryngology 医学-耳鼻喉科学

CiteScore

3.20

自引率

6.70%

发文量

276

审稿时长

62 days

期刊介绍： The purpose of the International Journal of Pediatric Otorhinolaryngology is to concentrate and disseminate information concerning prevention, cure and care of otorhinolaryngological disorders in infants and children due to developmental, degenerative, infectious, neoplastic, traumatic, social, psychiatric and economic causes. The Journal provides a medium for clinical and basic contributions in all of the areas of pediatric otorhinolaryngology. This includes medical and surgical otology, bronchoesophagology, laryngology, rhinology, diseases of the head and neck, and disorders of communication, including voice, speech and language disorders.