Correlation between antibacterial activity and compound composition of Ardisia mamillata Hance extract

IF 2 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemical Systematics and Ecology Pub Date : 2025-09-26 DOI:10.1016/j.bse.2025.105142

Lei Zeng , Yingle Chen , Wei Wei , Song Wang , Liu Yang , Qiaoguang Li , Zhihong Wang

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引用次数: 0

Abstract

This study investigates the antibacterial potential of extracts from the two different varieties of Ardisia mamillata Hance, a plant known for its medicinal value and natural adaptation to damp, fungal-prone environments. Although widely appreciated for its ornamental and traditional therapeutic uses, its antimicrobial activity remains underexplored. Extracts were prepared from red-leaf (Am_red) and green-leaf (Am_green) variants and tested against two phytopathogenic fungi: Fusarium and Botrytis cinerea. Both variants exhibited antifungal activity, with Am_red extracts showing superior efficacy—achieving a 33.4 % higher average inhibition rate compared to Am_green. In particular, Am_red extracts demonstrated strong inhibition against B. cinerea, with an average inhibition rate of 87.23 %.

UPLC-MS/MS analysis revealed that the dominant chemical classes in both extract types were flavonoids, lipids, amino acids and derivatives, alkaloids, phenolic acids, and organic acids—comprising 86.32 % in Am_red and 85.56 % in Am_green. Several bioactive compounds were identified, including petunidin-3-O-glucoside, calycosin, hispidulin, vanillic acid, and syringic acid. The preliminary data suggests that flavonoids, phenolic acids, and alkaloids are the key contributors to the antibacterial properties observed. The findings highlight the potential of A. mamillata Hance, particularly its red-leaf form, as a source of natural antimicrobial agents for sustainable applications in plant protection and phytopathogen control.

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荆芥提取物抑菌活性与复方成分的相关性研究

本研究研究了两种不同品种紫荆（Ardisia mamillata Hance）提取物的抗菌潜力，这种植物以其药用价值和对潮湿、真菌易发环境的天然适应性而闻名。虽然其观赏和传统治疗用途广受赞赏，但其抗菌活性仍未得到充分开发。从红叶（Am_red）和绿叶（Am_green）变体中提取提取物，并对两种植物病原真菌镰刀菌（Fusarium）和灰霉病菌（Botrytis cinerea）进行了抗性试验。两种变体均表现出抗真菌活性，其中Am_red提取物表现出更好的效果，平均抑制率比Am_green高33.4%。其中，Am_red提取物对灰霉病芽孢杆菌具有较强的抑制作用，平均抑制率为87.23%。UPLC-MS/MS分析表明，两种提取物的主要化学成分为黄酮类、脂类、氨基酸及其衍生物、生物碱、酚酸和有机酸，分别占Am_red和Am_green的86.32%和85.56%。鉴定出几种生物活性化合物，包括牵牛花苷-3- o -葡萄糖苷、毛蕊异黄酮、海鞘苷、香草酸和丁香酸。初步数据表明，黄酮类化合物、酚酸和生物碱是其抗菌性能的主要贡献者。这些发现突出了a . mamillata Hance的潜力，特别是其红叶形式，作为天然抗菌剂的来源，在植物保护和植物病原体控制方面具有可持续的应用。

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来源期刊

Biochemical Systematics and Ecology 生物-进化生物学

CiteScore

3.00

自引率

12.50%

发文量

147

审稿时长

43 days

期刊介绍： Biochemical Systematics and Ecology is devoted to the publication of original papers and reviews, both submitted and invited, in two subject areas: I) the application of biochemistry to problems relating to systematic biology of organisms (biochemical systematics); II) the role of biochemistry in interactions between organisms or between an organism and its environment (biochemical ecology). In the Biochemical Systematics subject area, comparative studies of the distribution of (secondary) metabolites within a wider taxon (e.g. genus or family) are welcome. Comparative studies, encompassing multiple accessions of each of the taxa within their distribution are particularly encouraged. Welcome are also studies combining classical chemosystematic studies (such as comparative HPLC-MS or GC-MS investigations) with (macro-) molecular phylogenetic studies. Studies that involve the comparative use of compounds to help differentiate among species such as adulterants or substitutes that illustrate the applied use of chemosystematics are welcome. In contrast, studies solely employing macromolecular phylogenetic techniques (gene sequences, RAPD studies etc.) will be considered out of scope. Discouraged are manuscripts that report known or new compounds from a single source taxon without addressing a systematic hypothesis. Also considered out of scope are studies using outdated and hard to reproduce macromolecular techniques such as RAPDs in combination with standard chemosystematic techniques such as GC-FID and GC-MS.