Quantification of intracellular mechanical fields in invasive cancer cells using digital volume correlation, confocal microscopy, and finite element method

IF 3.5 2区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of the Mechanical Behavior of Biomedical Materials Pub Date : 2025-09-23 DOI:10.1016/j.jmbbm.2025.107210

Aurélie Gangneux , Aymerick Gaboriau , Laetitia Caille , Marc Mesnil , Prasanth Bokam , Tanguy Vendeuvre , Stéphane Sebille , Norah Defamie , Arnaud Germaneau

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引用次数: 0

Abstract

Cell invasion process, which appears in the progression of tumours, such as glioblastoma, is highly dependent on cellular mobility. Cellular movement results from the interaction of chemical, biological and mechanical factors both inside and outside the invasive cancer cell. To identify and understand the relationship between these factors, it is necessary to quantify and visualise the extra- and intracellular kinematic fields during cell movement. This study proposes a new methodology for the experimental measurement of full kinematic fields inside cancer cells and the use of a digital twin simulation of the cell to obtain the stress and force fields. Confocal microscopy, Digital Volume Correlation (DVC) and Finite Element Method (FEM) are used in this methodology. To demonstrate the efficiency of this approach, highly invasive glioblastoma cells have been used as a model.

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利用数字体积相关、共聚焦显微镜和有限元法定量浸润性癌细胞的细胞内力学场

细胞侵袭过程，出现在肿瘤的进展，如胶质母细胞瘤，是高度依赖于细胞的流动性。细胞运动是侵袭性癌细胞内外化学、生物和机械因素相互作用的结果。为了识别和理解这些因素之间的关系，有必要量化和可视化细胞运动期间细胞外和细胞内的运动场。本研究提出了一种新的方法，用于实验测量癌细胞内的全运动学场，并使用细胞的数字孪生模拟来获得应力场和力场。该方法使用了共聚焦显微镜、数字体积相关（DVC）和有限元法（FEM）。为了证明这种方法的有效性，高侵袭性胶质母细胞瘤细胞被用作模型。

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来源期刊

Journal of the Mechanical Behavior of Biomedical Materials 工程技术-材料科学：生物材料

CiteScore

7.20

自引率

7.70%

发文量

505

审稿时长

46 days

期刊介绍： The Journal of the Mechanical Behavior of Biomedical Materials is concerned with the mechanical deformation, damage and failure under applied forces, of biological material (at the tissue, cellular and molecular levels) and of biomaterials, i.e. those materials which are designed to mimic or replace biological materials. The primary focus of the journal is the synthesis of materials science, biology, and medical and dental science. Reports of fundamental scientific investigations are welcome, as are articles concerned with the practical application of materials in medical devices. Both experimental and theoretical work is of interest; theoretical papers will normally include comparison of predictions with experimental data, though we recognize that this may not always be appropriate. The journal also publishes technical notes concerned with emerging experimental or theoretical techniques, letters to the editor and, by invitation, review articles and papers describing existing techniques for the benefit of an interdisciplinary readership.