Oral feeding of egg yolk antibody provide passive immune protection against largemouth bass iridovirus

IF 3.9 2区 农林科学 Q1 FISHERIES
Jinqiao Cao , Shijia Liu , Jiayi Luo , Xia Liang , Quan Wang , Zengjian Liang , Yunshang Ning , Tao Xu , Qiwei Qin , Sumei Xiao , Sheng Zhou
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引用次数: 0

Abstract

This study aimed to isolate water-soluble fraction (WSF) containing egg yolk immunoglobulin (IgY) from inactivated Largemouth bass (Micropterus salmoides) iridovirus (LMBV)-immunized egg yolks and assess their oral efficacy in preventing LMBV infection in largemouth bass. Through western blotting and indirect ELISA methods, it was observed that IgY specifically recognized major capsid protein (MCP), with its titer peaking at 8 weeks post-initial immunization and subsequently stabilizing. In vitro, antibody neutralization experiments demonstrated the ability of IgY to neutralize the virus and inhibit its replication. Animal experiments revealed a relative protection rate of approximately 54 % in the specific IgY group, accompanied by significantly reduced viral copy numbers, expression levels of inflammatory factors, and alleviated tissue pathological changes. Serum peptide detection indicated the presence of LMBV and IgY-related peptides circulating in the blood, which may contribute to virus neutralization. These findings suggest that polyclonal antibody IgY exhibits effective preventive properties against LMBV.
口服卵黄抗体对大口黑鲈虹膜病毒具有被动免疫保护作用
本研究旨在从灭活的大口黑鲈虹膜病毒(LMBV)免疫蛋黄中分离出含有蛋黄免疫球蛋白(IgY)的水溶性组分(WSF),并评价其对大口黑鲈虹膜病毒(LMBV)感染的口服预防效果。通过western blotting和间接ELISA方法观察到IgY特异性识别主要衣壳蛋白(MCP),其滴度在初次免疫后8周达到峰值,随后趋于稳定。在体外,抗体中和实验证明IgY能够中和病毒并抑制其复制。动物实验显示,特定IgY组的相对保护率约为54%,同时显著降低病毒拷贝数、炎症因子表达水平,减轻组织病理改变。血清肽检测显示血液中存在LMBV和igy相关肽循环,这可能有助于病毒中和。以上结果提示,IgY多克隆抗体对LMBV具有有效的预防作用。
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来源期刊
Fish & shellfish immunology
Fish & shellfish immunology 农林科学-海洋与淡水生物学
CiteScore
7.50
自引率
19.10%
发文量
750
审稿时长
68 days
期刊介绍: Fish and Shellfish Immunology rapidly publishes high-quality, peer-refereed contributions in the expanding fields of fish and shellfish immunology. It presents studies on the basic mechanisms of both the specific and non-specific defense systems, the cells, tissues, and humoral factors involved, their dependence on environmental and intrinsic factors, response to pathogens, response to vaccination, and applied studies on the development of specific vaccines for use in the aquaculture industry.
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