Recombinant Amuc_1100 Lactococcus lactis regulates metabolism and intestinal health of largemouth bass fed high starch diet

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture Pub Date : 2025-09-22 DOI:10.1016/j.aquaculture.2025.743232

Guokun Yang , Mingyue Yin , Shihui Guo , Boya Yang , Jianing Gu , Ying Zhang , Yanmin Zhang , Xindang Zhang , Xulu Chang , Shikun Feng , Xiaolin Meng

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引用次数: 0

Abstract

The present research focused on elucidating the functions of Amuc_1100 in largemouth bass. In vitro, the primary hepatocytes were treated by recombinant Amuc_1100 protein. In vivo, the roles of recombinant Amuc_1100 Lactococcus lactis was assessed on the largemouth bass induced by high starch diet. A total of 420 juvenile largemouth bass (20.87 ± 0.30 g) were distributed into 12 tanks (35 fish/ tank) for four groups: C group (9 % starch), H group (18 % starch), R group (18 % starch with MG1363), and A group (18 % starch with Am-MG1363). A 56-day feeding trial was conducted. The primary hepatocytes results indicate that the genes related to glucose and lipid metabolism were regulated by Amuc_1100 protein. In vivo, the FBW, WGR, SGR and CF were no statistically significant differences in different groups (P > 0.05). The serum GHb, TG, TC, LDL-C, ALT and AST levels were markedly decreased in A group compared to the H group (P < 0.05). Furthermore, INS content was dramatically promoted in A group compared to the H group in the 8th week (P < 0.05). In liver tissue, the hepatic glycogen, lipid droplets were dramatically decreased in A group compared to the H group (P < 0.05). Moreover, pk, pygl, fas and acc-α expression were dramatically decreased (P < 0.05), lpl and hsl expression were markedly promoted (P < 0.05) in A group compared to the H group. In intestinal tissue, inflammatory infiltration induced by high starch diet was attenuated in the A group. In addition, the caspase3, caspase9, tnf-α and il-8 expression were markedly inhibited (P < 0.05), zo-1 and il-10 expression were observably promoted (P < 0.05) in the A group compared to the H group. The IL-10 and TGF-β contents were dramatically promoted (P < 0.05), and TNF-α level was markedly decreased (P < 0.05) in the A group. In intestinal contents, the acetic acid and total SCFAs contents were markedly elevated in A group compared to the H group (P < 0.05). Gut microbiota composition was dramatically altered in A group compared to the H group. These results will provide theoretical basis for application of Amuc_1100 in aquaculture.

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重组Amuc_1100乳酸乳球菌对高淀粉饲料大口黑鲈代谢和肠道健康的调节作用

目前的研究重点是阐明Amuc_1100在大口黑鲈中的功能。体外用重组Amuc_1100蛋白处理原代肝细胞。在体内，研究了重组Amuc_1100乳酸乳球菌对高淀粉饲料诱导的大口黑鲈的作用。选取420尾（20.87±0.30 g）大嘴黑鲈幼鱼，分成12个鱼缸（35尾/个），分为4组：C组（淀粉含量9%）、H组（淀粉含量18%）、R组（淀粉含量18%）和A组（淀粉含量18%）。进行56 d饲养试验。原代肝细胞实验结果表明，糖脂代谢相关基因受Amuc_1100蛋白调控。体内各组体增重、增重率、增重率和CF比较，差异均无统计学意义（P > 0.05）。与H组相比，A组血清GHb、TG、TC、LDL-C、ALT、AST水平均显著降低（P < 0.05）。第8周，A组INS含量较H组显著提高（P < 0.05）。肝组织中，A组肝糖原、脂滴较H组显著减少（P < 0.05）。与H组相比，A组pk、pygl、fas和acc-α的表达显著降低（P < 0.05）， lpl和hsl的表达显著升高（P < 0.05）。在肠道组织中，A组高淀粉日粮引起的炎症浸润减弱。此外，与H组相比，A组caspase3、caspase9、tnf-α、il-8的表达明显受到抑制（P < 0.05）， zo-1、il-10的表达明显升高（P < 0.05）。A组IL-10、TGF-β含量显著升高（P < 0.05）， TNF-α水平显著降低（P < 0.05）。肠道内容物中，A组乙酸和总短链脂肪酸含量显著高于H组（P < 0.05）。与H组相比，A组肠道菌群组成发生了显著变化。这些结果将为Amuc_1100在水产养殖中的应用提供理论依据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Aquaculture 农林科学-海洋与淡水生物学

CiteScore

8.60

自引率

17.80%

发文量

1246

审稿时长

56 days

期刊介绍： Aquaculture is an international journal for the exploration, improvement and management of all freshwater and marine food resources. It publishes novel and innovative research of world-wide interest on farming of aquatic organisms, which includes finfish, mollusks, crustaceans and aquatic plants for human consumption. Research on ornamentals is not a focus of the Journal. Aquaculture only publishes papers with a clear relevance to improving aquaculture practices or a potential application.