Aptamer-based biosensors for metal ion and mycotoxin detection: Principles and progress

IF 4.2 3区工程技术 Q2 CHEMISTRY, APPLIED

Dyes and Pigments Pub Date : 2025-09-23 DOI:10.1016/j.dyepig.2025.113272

Lu Li , Yilong Li , Xinyan Chen , Chaoran Lei , Miao Lin , Houyong Luo , Pan Xie , Juan Wei , Guiqiang Fei

{"title":"Aptamer-based biosensors for metal ion and mycotoxin detection: Principles and progress","authors":"Lu Li , Yilong Li , Xinyan Chen , Chaoran Lei , Miao Lin , Houyong Luo , Pan Xie , Juan Wei , Guiqiang Fei","doi":"10.1016/j.dyepig.2025.113272","DOIUrl":null,"url":null,"abstract":"<div><div>Heavy metal ions and mycotoxins pose serious risks to food safety and public health. Conventional analytical methods such as chromatography and mass spectrometry are accurate but remain costly, labor-intensive, and unsuitable for rapid on-site monitoring. Aptamers, synthetic single-stranded DNA or RNA obtained through SELEX, provide high affinity, good stability, and easy modification, making them attractive recognition elements for biosensor design. This review highlights recent progress in aptamer-based biosensors for detecting toxic metals (Hg<sup>2+</sup>, Pb<sup>2+</sup>, Cd<sup>2+</sup>, As<sup>3+</sup>, Ag<sup>+</sup>, Cu<sup>2+</sup>, Mn<sup>2+</sup>) and mycotoxins (OTA, AFB1, ZEN, DON, FB1). With the aid of nanomaterials, optical and electrochemical transduction, and molecular amplification, many sensors have achieved ultra-low detection limits from nanomolar to femtomolar levels, together with improved selectivity and performance in complex matrices. Current challenges include limited availability of high-affinity aptamers, matrix interference, and lack of standardized protocols. Future advances are expected through high-throughput aptamer screening, multimodal sensing strategies, and integration with portable microfluidic or paper-based devices to promote applications.</div></div>","PeriodicalId":302,"journal":{"name":"Dyes and Pigments","volume":"245 ","pages":"Article 113272"},"PeriodicalIF":4.2000,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Dyes and Pigments","FirstCategoryId":"88","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0143720825006424","RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}

引用次数: 0

Abstract

Heavy metal ions and mycotoxins pose serious risks to food safety and public health. Conventional analytical methods such as chromatography and mass spectrometry are accurate but remain costly, labor-intensive, and unsuitable for rapid on-site monitoring. Aptamers, synthetic single-stranded DNA or RNA obtained through SELEX, provide high affinity, good stability, and easy modification, making them attractive recognition elements for biosensor design. This review highlights recent progress in aptamer-based biosensors for detecting toxic metals (Hg²⁺, Pb²⁺, Cd²⁺, As³⁺, Ag⁺, Cu²⁺, Mn²⁺) and mycotoxins (OTA, AFB1, ZEN, DON, FB1). With the aid of nanomaterials, optical and electrochemical transduction, and molecular amplification, many sensors have achieved ultra-low detection limits from nanomolar to femtomolar levels, together with improved selectivity and performance in complex matrices. Current challenges include limited availability of high-affinity aptamers, matrix interference, and lack of standardized protocols. Future advances are expected through high-throughput aptamer screening, multimodal sensing strategies, and integration with portable microfluidic or paper-based devices to promote applications.

Abstract Image

查看原文本刊更多论文

基于适配体的金属离子和真菌毒素检测生物传感器：原理与进展

重金属离子和真菌毒素对食品安全和公众健康构成严重威胁。传统的分析方法，如色谱法和质谱法是准确的，但仍然昂贵，劳动密集型，不适合快速现场监测。适配体是通过SELEX获得的合成单链DNA或RNA，具有高亲和力、良好的稳定性和易于修饰的特点，是生物传感器设计中有吸引力的识别元件。本文综述了基于适体的生物传感器在检测有毒金属（Hg2+, Pb2+, Cd2+, As3+, Ag+, Cu2+, Mn2+）和真菌毒素（OTA, AFB1， ZEN， DON, FB1）方面的最新进展。在纳米材料、光学和电化学转导以及分子扩增的帮助下，许多传感器已经实现了从纳摩尔到飞摩尔水平的超低检测限，同时提高了在复杂基质中的选择性和性能。目前的挑战包括高亲和力适配体的有限可用性、基质干扰和缺乏标准化协议。未来的进展有望通过高通量适体筛选，多模态传感策略，以及与便携式微流体或纸质设备的集成来促进应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Dyes and Pigments 工程技术-材料科学：纺织

CiteScore

8.20

自引率

13.30%

发文量

933

审稿时长

33 days

期刊介绍： Dyes and Pigments covers the scientific and technical aspects of the chemistry and physics of dyes, pigments and their intermediates. Emphasis is placed on the properties of the colouring matters themselves rather than on their applications or the system in which they may be applied. Thus the journal accepts research and review papers on the synthesis of dyes, pigments and intermediates, their physical or chemical properties, e.g. spectroscopic, surface, solution or solid state characteristics, the physical aspects of their preparation, e.g. precipitation, nucleation and growth, crystal formation, liquid crystalline characteristics, their photochemical, ecological or biological properties and the relationship between colour and chemical constitution. However, papers are considered which deal with the more fundamental aspects of colourant application and of the interactions of colourants with substrates or media. The journal will interest a wide variety of workers in a range of disciplines whose work involves dyes, pigments and their intermediates, and provides a platform for investigators with common interests but diverse fields of activity such as cosmetics, reprographics, dye and pigment synthesis, medical research, polymers, etc.