Evaluation of the role of age in MMP 9 and MMP 2 levels in pulpal blood of teeth with symptomatic irreversible pulpitis

Q1 Medicine

Journal of oral biology and craniofacial research Pub Date : 2025-09-27 DOI:10.1016/j.jobcr.2025.09.022

Praneeth rednam , Sarath sarathy

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引用次数: 0

Abstract

Aim

To assess MMP 2 and MMP 9 levels in pulpal blood of human molar teeth with symptomatic irreversible pulpitis in 2 different age groups.

Materials and methods

Patients of two age groups young [18–40] and old [>60] with symptomatic irreversible pulpitis in molars were included in this study. Teeth were isolated using a rubber dam. The rubber dam, clamp, and tooth were decontaminated using a cotton swab drenched in 1 % NaOCl solution. Access cavities were then prepared using a diamond-coated bur in a contrangle handpiece under water-cooling. Subsequently, the caries was excavated using a sterile round bur. Pulpal blood was collected from the pulp chamber by placing the blunt end of a sterile inverted 60 size paper point for 1 min. The paper point was transferred into a sterile microcentrifugation tube and frozen at −20 °C immediately until further processing. The levels of MMP-9 and MMP-2 in these samples were measured using commercially available specific enzyme-linked immunosorbent assay (ELISA) kits. Data was analysed using an independent t-test.

Results

The mean pulpal blood levels of MMP-9 in patients above 60 years (220 ng/ml) were significantly lower than those aged 18–40 years (468 ng/ml) ([p value < 0.05]

The mean pulpal blood levels of MMP-2 in patients above 60 years (134 ng/ml) were significantly lower than in those aged 18–40 years (153 ng/ml [p value < 0.05].

Conclusion

MMP-9 and MMP-2 levels in pulpal blood of cariously exposed teeth with irreversible pulpitis can vary with age.

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年龄对症状性不可逆性牙髓炎牙髓血中mmp9和mmp2水平的影响

目的评价2个不同年龄组有症状的不可逆性牙髓炎患者磨牙髓血中mmp2和mmp9的水平。材料与方法本研究分为青年[18-40]和老年[>；60]两组有症状的磨牙不可逆性牙髓炎患者。牙齿用橡胶坝隔离。用浸有1% NaOCl溶液的棉签对橡胶坝、夹钳和牙进行消毒。然后，在水冷却的情况下，在一个对角机头中使用涂有金刚石的bur制备通道腔。随后，使用无菌圆钻挖掘龋。从髓腔收集髓血，将钝端放置在一个无菌倒置60大小的纸点上1分钟。将纸点转移到无菌微离心管中，立即在- 20°C冷冻以待进一步处理。这些样品中MMP-9和MMP-2的水平使用市售的特异性酶联免疫吸附测定（ELISA）试剂盒进行测定。数据分析采用独立t检验。结果60岁以上患者髓血MMP-9平均水平（220 ng/ml）显著低于18-40岁患者（468 ng/ml）（p值<； 0.05）， 60岁以上患者髓血MMP-2平均水平（134 ng/ml）显著低于18-40岁患者（153 ng/ml）（p值<； 0.05）。结论龋露牙不可逆牙髓炎患者牙髓血中mmp -9、MMP-2水平随年龄变化而变化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of oral biology and craniofacial research Medicine-Otorhinolaryngology

CiteScore

4.90

自引率

0.00%

发文量

133

审稿时长

167 days

期刊介绍： Journal of Oral Biology and Craniofacial Research (JOBCR)is the official journal of the Craniofacial Research Foundation (CRF). The journal aims to provide a common platform for both clinical and translational research and to promote interdisciplinary sciences in craniofacial region. JOBCR publishes content that includes diseases, injuries and defects in the head, neck, face, jaws and the hard and soft tissues of the mouth and jaws and face region; diagnosis and medical management of diseases specific to the orofacial tissues and of oral manifestations of systemic diseases; studies on identifying populations at risk of oral disease or in need of specific care, and comparing regional, environmental, social, and access similarities and differences in dental care between populations; diseases of the mouth and related structures like salivary glands, temporomandibular joints, facial muscles and perioral skin; biomedical engineering, tissue engineering and stem cells. The journal publishes reviews, commentaries, peer-reviewed original research articles, short communication, and case reports.