Commensal Bacillus altitudinis F7-derived cell wall constituents alleviated high starch diet-induced glycolipid metabolism disorders and liver injury in Micropterus salmoides

IF 2.7 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Feed Science and Technology Pub Date : 2025-09-20 DOI:10.1016/j.anifeedsci.2025.116509

Bi-Yun Zhang , Hong-Ling Yang , Guo-He Cai , Zhi-Qing Qin , Yun-Zhang Sun

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引用次数: 0

Abstract

Commensal Bacillus altitudinis F7 had probiotic effects on high starch diet fed largemouth bass (Micropterus salmoides), but it was not known whether its cell wall constituents (peptidoglycan (PGN), lipoteichoic acid (LTA) and whole cell wall (WCW)) were the main contributors. To elucidate this, low starch (6 %, LS) and high starch (18 %, HS) diets were respectively designed as positive and negative controls, and PGN, LTA and WCW were supplemented alone to HS diet to determine their potential influences on M. salmoides regarding growth performance, glycolipid metabolism, and liver health. The results manifested that PGN, LTA and WCW relieved the HS diet-induced growth retardation and glycolipid metabolism disorders via reducing the activities of hepatic enzymes engaged in glycolysis and fat synthesis, improving the activities of hepatic enzymes engaged in gluconeogenesis and lipolysis, thereby reducing the serum glucose content, hepatic glycogen content, serum total cholesterol (TC) and triglyceride (TG) levels, and hepatic and muscle fat contents. Additionally, PGN, LTA and WCW activated insulin and FXR/SHP signaling pathways, regulated glycolysis, gluconeogenesis and lipid metabolism. PGN, LTA and WCW enhanced the liver health by improving the hepatic function, reducing inflammation, and decreasing the number of vacuoles and lipid droplets. In conclusion, PGN, LTA and WCW of heat-inactivated B. altitudinis F7 showed beneficial potentials in improving the glycolipid metabolism, thus promoting growth and liver health in HS diet fed M. salmoides, and among which the PGN showed the best efficacy. This study supplies theoretical bases for revealing the cell wall constituents as the determining factors for B. altitudinis F7 exerting probiotic functions, and also for the postbiotics application in improving glycolipid metabolism in fish fed high starch diet.

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共生高原芽孢杆菌f7来源的细胞壁成分可减轻高淀粉饮食诱导的小鲵糖脂代谢紊乱和肝损伤

共生高原芽孢杆菌F7对高淀粉饲料喂养的大口黑鲈（Micropterus salmoides）有益生菌作用，但其细胞壁成分(肽聚糖（PGN）、脂质胆酸（LTA）和全细胞壁（WCW）是否为主要作用因子尚不清楚。为此，分别设计低淀粉（6 %，LS）和高淀粉（18 %，HS）饲粮作为阳性对照和阴性对照，并在HS饲粮中单独添加PGN、LTA和WCW，以确定它们对salmoides生长性能、糖脂代谢和肝脏健康的潜在影响。结果表明，PGN、LTA和WCW通过降低糖酵解和脂肪合成肝酶活性，提高糖异生和脂肪分解肝酶活性，从而降低血清葡萄糖含量、肝糖原含量、血清总胆固醇（TC）和甘油三酯（TG）水平，以及肝脏和肌肉脂肪含量，缓解了HS日粮诱导的生长迟缓和糖脂代谢紊乱。此外，PGN、LTA和WCW激活胰岛素和FXR/SHP信号通路，调节糖酵解、糖异生和脂质代谢。PGN、LTA和WCW通过改善肝功能、减轻炎症、减少空泡和脂滴数量来改善肝脏健康。综上所述，热灭活高原白僵菌F7的PGN、LTA和WCW均具有改善沙门沙棘糖脂代谢、促进生长和肝脏健康的有利潜力，其中PGN的效果最好。本研究为揭示高海拔双歧杆菌F7发挥益生菌功能的决定因素是细胞壁成分提供了理论依据，也为提高高淀粉饲料中糖脂代谢的生后制剂应用提供了理论依据。

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来源期刊

Animal Feed Science and Technology 农林科学-奶制品与动物科学

CiteScore

6.00

自引率

6.20%

发文量

266

审稿时长

3 months

期刊介绍： Animal Feed Science and Technology is a unique journal publishing scientific papers of international interest focusing on animal feeds and their feeding. Papers describing research on feed for ruminants and non-ruminants, including poultry, horses, companion animals and aquatic animals, are welcome. The journal covers the following areas: Nutritive value of feeds (e.g., assessment, improvement) Methods of conserving and processing feeds that affect their nutritional value Agronomic and climatic factors influencing the nutritive value of feeds Utilization of feeds and the improvement of such Metabolic, production, reproduction and health responses, as well as potential environmental impacts, of diet inputs and feed technologies (e.g., feeds, feed additives, feed components, mycotoxins) Mathematical models relating directly to animal-feed interactions Analytical and experimental methods for feed evaluation Environmental impacts of feed technologies in animal production.