A ratiometric NIR fluorescent probe for simultaneous detection of H2S and viscosity: Applications in environmental monitoring and bioimaging

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open Pub Date : 2025-09-23 DOI:10.1016/j.talo.2025.100564

Sha Li, Qin Li, Zhihui Yang, Wei Wen, Miao Yan, Haixian Ren

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Abstract

Hydrogen sulfide (H₂S), a critical gasotransmitter governing physiological functions, presents significant environmental and food safety hazards at aberrant concentrations. Concurrently, mitochondrial viscosity serves as a vital biomarker for cellular pathologies. To address the unmet need for precise dual-parameter monitoring, we engineered a mitochondria-targeted near-infrared (NIR) ratiometric fluorescent probe, T-N, enabling simultaneous and discriminative detection of H₂S and viscosity through discrete emission channels. The probe exhibits ultrarapid response (<30 s), exceptional sensitivity, and superb selectivity against competing analytes. Leveraging H₂S-triggered chromogenic shifts, T-N-immobilized test strips facilitate instrument-free, semi-quantitative assessment of food spoilage, validated across environmental waters and industrially relevant beer samples. Moreover, T-N achieves synchronous dual-channel imaging of endogenous/exogenous H₂S and viscosity dynamics in live cells, with verified mitochondrial localization. This work establishes a versatile sensing platform for tracking H₂S and viscosity in interconnected environmental, food safety, and biomedical contexts.

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同时检测H2S和黏度的比例近红外荧光探针：在环境监测和生物成像中的应用

硫化氢（H2S）是一种控制生理功能的重要气体递质，在异常浓度下会对环境和食品安全造成重大危害。同时，线粒体粘度作为细胞病理的重要生物标志物。为了满足精确双参数监测的需求，我们设计了一种针对线粒体的近红外（NIR）比例荧光探针T-N，可以通过离散发射通道同时鉴别检测H2S和粘度。该探针具有超快速响应（30秒），卓越的灵敏度和对竞争分析物的极好选择性。利用h2s触发的显色变化，t- n固定试纸可以实现无仪器、半定量的食品腐败评估，并在环境水域和工业相关的啤酒样品中得到验证。此外，T-N在活细胞中实现了内源性/外源性H2S和黏度动力学的同步双通道成像，并验证了线粒体定位。这项工作建立了一个多功能传感平台，用于在相互关联的环境、食品安全和生物医学环境中跟踪H2S和粘度。

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