Portable histone assay technology (PHAST) for the sample-to-answer detection of circulating histones in whole blood

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical Pub Date : 2025-09-27 DOI:10.1016/j.snb.2025.138848

Rachel L. Warren, John R. Rzasa, Zehra F. Syeda, Alexander R. Pueschel, Ian M. White

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引用次数: 0

Abstract

Circulating histones are a potential biomarker for organ failure, organ transplant rejection, and sepsis. There is a need for point-of-care detection of histones in patient samples to enable diagnostics in the emergency department (sepsis), military field hospitals (trauma-induced organ failure), and at home (transplant rejection). For these applications, it is necessary to detect histones directly from whole blood samples without any intermediate steps and with low-cost portable instrumentation. Here we introduce portable histone assay technology (PHAST), an assay and a low-cost handheld instrument for the sample-to-answer detection of histones directly from blood samples. The assay utilizes a DNA-based FRET reporter molecule to enable direct detection of histones in a single step, while anti-red-blood-cell magnetic beads in combination with a moveable magnet in the handheld instrument remove the red blood cells prior to the fluorescence measurement. Using this novel approach, we detect clinically relevant concentrations of histones spiked into whole blood in under 20 minutes.

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便携式组蛋白测定技术（PHAST）用于全血中循环组蛋白的样本到应答检测

循环组蛋白是器官衰竭、器官移植排斥反应和败血症的潜在生物标志物。有必要对患者样本中的组蛋白进行即时检测，以便在急诊科（败血症）、军事野战医院（创伤性器官衰竭）和家庭（移植排斥）进行诊断。对于这些应用，有必要直接从全血样本中检测组蛋白，而不需要任何中间步骤，并且使用低成本的便携式仪器。在这里，我们介绍便携式组蛋白检测技术（PHAST），这是一种低成本的手持式仪器，用于直接从血液样本中检测组蛋白。该检测利用基于dna的FRET报告分子，能够在一个步骤中直接检测组蛋白，而抗红细胞磁珠与手持式仪器中的可移动磁铁相结合，在荧光测量之前去除红细胞。使用这种新方法，我们可以在20分钟内检测到全血中与临床相关的组蛋白浓度。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Sensors and Actuators B: Chemical 工程技术-电化学

CiteScore

14.60

自引率

11.90%

发文量

1776

审稿时长

3.2 months

期刊介绍： Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.