Molecular architecture of developing xylem in Norway spruce reveals spatial and temporal patterns of cell wall polymer deposition.

IF 5.7 2区 生物学 Q1 PLANT SCIENCES
Olga Blokhina, Yaseen Mottiar, Notburga Gierlinger, Catherine Jones, William Willats, Kurt Fagerstedt, Anna Kärkonen
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引用次数: 0

Abstract

Using microarray polymer profiling (MAPP), immunocytochemical staining, and Raman spectroscopy, we conducted a detailed spatiotemporal survey of the deposition of cell wall components during xylem differentiation in Norway spruce. These insights are essential for understanding the intricate architecture of wood cell walls during the initial stages of development immediately following cambial divisions. MAPP was used to obtain an overview of cell wall polysaccharides, glycoproteins, and ferulic acid residues in developing xylem. The most interesting findings were investigated in further detail using immunomicroscopy and Raman spectroscopy. In axial tracheids, deposition of pectic compounds and cellulose only slightly preceded the first deposition of lignin. Lignification was initiated in the middle lamellae and cell wall corners and proceeded during the development of the S1 layer and intensified later during cell wall thickening. Raman analysis revealed an earlier deposition of lignin than shown previously, which coincided with the deposition of methylesterified or partially methylesterified homogalacturonan initially in the radial cell walls and later in the tangential cell walls of developing earlywood. Minimal binding of an antibody against feruloylated compounds indicated that ferulic acid residues do not participate in the initiation of lignification in Norway spruce.

挪威云杉发育木质部的分子结构揭示了细胞壁聚合物沉积的时空格局。
利用微阵列聚合物图谱(MAPP)、免疫细胞化学染色和拉曼光谱技术,对挪威云杉木质部分化过程中细胞壁成分的沉积进行了详细的时空调查。这些见解对于理解在形成层分裂后的初始发育阶段木材细胞壁的复杂结构至关重要。MAPP应用于木质部发育过程中细胞壁多糖、糖蛋白和阿魏酸残基的研究。使用免疫显微镜和拉曼光谱对最有趣的发现进行了进一步的详细研究。在轴向管胞中,果胶化合物和纤维素的沉积仅略早于木质素的首次沉积。木质化始于中间片层和细胞壁角,并在S1层发育过程中进行,随后在细胞壁增厚过程中加强。拉曼分析显示木质素的沉积比之前所示的要早,这与甲基化或部分甲基化的均半乳糖醛酸的沉积相吻合,最初在放射状细胞壁,后来在发育早期的切向细胞壁。针对阿魏酸化化合物的抗体的最小结合表明,阿魏酸残基不参与挪威云杉木质素化的起始。
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来源期刊
Journal of Experimental Botany
Journal of Experimental Botany 生物-植物科学
CiteScore
12.30
自引率
4.30%
发文量
450
审稿时长
1.9 months
期刊介绍: The Journal of Experimental Botany publishes high-quality primary research and review papers in the plant sciences. These papers cover a range of disciplines from molecular and cellular physiology and biochemistry through whole plant physiology to community physiology. Full-length primary papers should contribute to our understanding of how plants develop and function, and should provide new insights into biological processes. The journal will not publish purely descriptive papers or papers that report a well-known process in a species in which the process has not been identified previously. Articles should be concise and generally limited to 10 printed pages.
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