{"title":"Identification, antifungal resistance, and genomic characterization of a single <i>Candida auris</i> isolate from urinary tract infection.","authors":"Jie Li, Ziheng Wang, Rui Zheng, Yangyan Wang, Xin Guo, Xiaoning Li, Peng Zhang","doi":"10.3389/fcimb.2025.1641542","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To analyze the identification, antifungal resistance, and genomic characteristics of a <i>Candida auris</i> (<i>C. auris</i>) strain isolated from a urine specimen of an ICU patient at Yijishan Hospital, Wannan Medical College, Anhui Province.</p><p><strong>Methods: </strong>The isolate was identified by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS). The susceptibility of the isolates to fungi was determined by measuring the Minimum Inhibitory Concentration (MIC) values using the VITEK 2 COMPACT system. Whole-genome sequencing (WGS) was performed using high-throughput technology. Resistance and virulence genes were annotated using public databases, including NCBI (https://www.ncbi.nlm.nih.gov/, version 2.2.28), DFVF (http://sysbio.unl.edu/DFVF/, version 1.0), PHI-base (http://www.phi-base.org, version 5.0) and KEGG (https://www.kegg.jp/, version 89.1). A phylogenetic tree was constructed through analysis of the 18S rRNA nucleotide sequence.</p><p><strong>Results: </strong>The isolate named CAS20503 was identified as <i>C. auris</i>. Antifungal susceptibility testing showed resistance to fluconazole and amphotericin B. Genomic analysis identified resistance genes including <i>ERG11</i> (azole), <i>FKS1</i> (echinocandin), <i>ERG3</i> (polyene), and efflux pumps <i>CDR1</i>, <i>MDR1</i>. Resistance mutations were detected. The virulence genes analyzed based on the DFVF database included <i>CaNik1</i>, <i>CHS2</i>, <i>DUR1,2</i>, <i>HSP90</i>, <i>ICL1</i>, <i>PMT1</i>, <i>PMT2</i>, <i>PMT4</i>, <i>SSD1</i>, <i>TPS2</i>. The host pathogenic genes identified by comparison with the PHI-base database included <i>CaCHS1, ADE2, FAS2, PMR1, CaTPS2, Tfp1.</i> KEGG annotation showed enrichment in infectious disease pathways. The phylogenetic tree constncted based on the nudeotide sequence analysis of 18S rRNA indicated that this strain (Genome accession number: JBPYFS000000000) exhibited a high degree of genomic similarity to the <i>C. auris</i> strain (Genome accession number: CP157510.1), which was isolated in ltaly in 2024 and belonged to clade l (a subset of the South Asian clade).</p><p><strong>Conclusion: </strong>Through an in-depth analysis of strain CAS20503, which was isolated from a urinary tract infection specimen at a tertiary public hospital in Anhui Province (Yijishan Hospital, Wannan Medical College), this study elucidated the drug resistance profiles and genomic characteristics of <i>C. auris</i>. The findings have provided critical evidence for the early identification, diagnosis, and optimization of antifungal therapeutic regimens for infections caused by this pathogen in clinical practice.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":"15 ","pages":"1641542"},"PeriodicalIF":4.8000,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12457345/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in Cellular and Infection Microbiology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3389/fcimb.2025.1641542","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"IMMUNOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: To analyze the identification, antifungal resistance, and genomic characteristics of a Candida auris (C. auris) strain isolated from a urine specimen of an ICU patient at Yijishan Hospital, Wannan Medical College, Anhui Province.
Methods: The isolate was identified by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS). The susceptibility of the isolates to fungi was determined by measuring the Minimum Inhibitory Concentration (MIC) values using the VITEK 2 COMPACT system. Whole-genome sequencing (WGS) was performed using high-throughput technology. Resistance and virulence genes were annotated using public databases, including NCBI (https://www.ncbi.nlm.nih.gov/, version 2.2.28), DFVF (http://sysbio.unl.edu/DFVF/, version 1.0), PHI-base (http://www.phi-base.org, version 5.0) and KEGG (https://www.kegg.jp/, version 89.1). A phylogenetic tree was constructed through analysis of the 18S rRNA nucleotide sequence.
Results: The isolate named CAS20503 was identified as C. auris. Antifungal susceptibility testing showed resistance to fluconazole and amphotericin B. Genomic analysis identified resistance genes including ERG11 (azole), FKS1 (echinocandin), ERG3 (polyene), and efflux pumps CDR1, MDR1. Resistance mutations were detected. The virulence genes analyzed based on the DFVF database included CaNik1, CHS2, DUR1,2, HSP90, ICL1, PMT1, PMT2, PMT4, SSD1, TPS2. The host pathogenic genes identified by comparison with the PHI-base database included CaCHS1, ADE2, FAS2, PMR1, CaTPS2, Tfp1. KEGG annotation showed enrichment in infectious disease pathways. The phylogenetic tree constncted based on the nudeotide sequence analysis of 18S rRNA indicated that this strain (Genome accession number: JBPYFS000000000) exhibited a high degree of genomic similarity to the C. auris strain (Genome accession number: CP157510.1), which was isolated in ltaly in 2024 and belonged to clade l (a subset of the South Asian clade).
Conclusion: Through an in-depth analysis of strain CAS20503, which was isolated from a urinary tract infection specimen at a tertiary public hospital in Anhui Province (Yijishan Hospital, Wannan Medical College), this study elucidated the drug resistance profiles and genomic characteristics of C. auris. The findings have provided critical evidence for the early identification, diagnosis, and optimization of antifungal therapeutic regimens for infections caused by this pathogen in clinical practice.
期刊介绍：
Frontiers in Cellular and Infection Microbiology is a leading specialty journal, publishing rigorously peer-reviewed research across all pathogenic microorganisms and their interaction with their hosts. Chief Editor Yousef Abu Kwaik, University of Louisville is supported by an outstanding Editorial Board of international experts. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide.
Frontiers in Cellular and Infection Microbiology includes research on bacteria, fungi, parasites, viruses, endosymbionts, prions and all microbial pathogens as well as the microbiota and its effect on health and disease in various hosts. The research approaches include molecular microbiology, cellular microbiology, gene regulation, proteomics, signal transduction, pathogenic evolution, genomics, structural biology, and virulence factors as well as model hosts. Areas of research to counteract infectious agents by the host include the host innate and adaptive immune responses as well as metabolic restrictions to various pathogenic microorganisms, vaccine design and development against various pathogenic microorganisms, and the mechanisms of antibiotic resistance and its countermeasures.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
