Highly selective 1,4-NADH regeneration via hydrogenated NAD+ analogs using a Pt/SiO2 catalyst with low pressure hydrogen gas.

Abstract

The hydrogenation of nicotinamide analogs using a Pt/SiO₂ catalyst, followed by hydride transfer to NAD⁺, results in the highly selective regeneration of 1,4-NADH. N-Methyl nicotinamide exhibits the highest reactivity, achieving 98% selectivity at 20 °C under 1 atm H₂. This strategy provides a practical and sustainable pathway for 1,4-NADH regeneration.