Özge Ata, Lisa Lutz, Michael Baumschabl, Diethard Mattanovich
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引用次数: 0
Abstract
Single carbon (C1) substrates are gaining importance as future feedstocks for the production of bio-based chemicals. Carbon dioxide, a major greenhouse gas, offers a promising alternative to the traditional feedstocks to shift towards C1-based, sustainable processes. Here, we present a synthetic autotrophic Komagataella phaffii (Pichia pastoris) that is able to produce itaconic acid by the direct conversion of CO2, achieving final titers of approximately 12 g L-1 in bioreactor cultivations. We show that a combined approach that integrates balancing the flux between the Calvin-Benson-Bassham (CBB) cycle and itaconic acid metabolism with process design was essential to enhance the production. Our study demonstrates the potential of K. phaffii as a microbial platform using CO2 as the direct carbon source, aligning with the future goals of establishing sustainable bioprocesses.
单碳（C1）底物作为未来生产生物基化学品的原料越来越重要。二氧化碳是一种主要的温室气体，为传统原料转向基于c1的可持续过程提供了一种有希望的替代方案。在这里，我们提出了一种合成的自养法菲Komagataella（毕赤酵母），它能够通过直接转化二氧化碳产生衣康酸，在生物反应器培养中达到约12 g L-1的最终滴度。研究表明，结合Calvin-Benson-Bassham （CBB）循环和衣康酸代谢之间的通量平衡以及工艺设计的综合方法对于提高产量至关重要。我们的研究证明了菲氏K. phaffii作为一个微生物平台的潜力，利用二氧化碳作为直接碳源，与建立可持续生物过程的未来目标一致。
期刊介绍：
Green Chemistry is a journal that provides a unique forum for the publication of innovative research on the development of alternative green and sustainable technologies. The scope of Green Chemistry is based on the definition proposed by Anastas and Warner (Green Chemistry: Theory and Practice, P T Anastas and J C Warner, Oxford University Press, Oxford, 1998), which defines green chemistry as the utilisation of a set of principles that reduces or eliminates the use or generation of hazardous substances in the design, manufacture and application of chemical products. Green Chemistry aims to reduce the environmental impact of the chemical enterprise by developing a technology base that is inherently non-toxic to living things and the environment. The journal welcomes submissions on all aspects of research relating to this endeavor and publishes original and significant cutting-edge research that is likely to be of wide general appeal. For a work to be published, it must present a significant advance in green chemistry, including a comparison with existing methods and a demonstration of advantages over those methods.
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