Identification and Characterization of the Starch Granule-Associated Proteins of Cereal Starches after Alkali Treatment

IF 5.4 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biomacromolecules Pub Date : 2025-09-26 DOI:10.1021/acs.biomac.5c01158

Zekun Xu, , , Zhiqian Wu, , , Xinyu Zhang, , , Mengting Ma, , , Zhongquan Sui*, , and , Harold Corke,

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Abstract

Starch granule-associated proteins (SGAPs) of four cereal starches subjected to different alkali treatments were investigated. Starch synthesis-related proteins were identified as the major SGAPs in normal and waxy maize starches. In rice starch, seed storage proteins were the majority of SGAPs. A concentric ring pattern of the SGAPs distribution was found in normal maize starch and normal rice starch, related to the granule-bound starch synthases. SGAPs of waxy maize starch mainly occur in starch channels, identified as starch synthases and starch branching enzymes. Alkali treatment decreased SGAPs by facilitating protein solubility and degrading the protein structure. Hydrothermal treatment promoted the effect of the alkali. After alkali treatment, the proportion of starch synthesis-related proteins in total SGAPs significantly increased, indicating that these were more resistant to alkali, as they were tightly bound to starch granules. This study revealed the heterogeneity of SGAPs in starch granules and their response to the alkaline treatment.

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碱处理后谷物淀粉淀粉粒相关蛋白的鉴定与表征

研究了四种谷物淀粉经不同碱处理后的淀粉颗粒相关蛋白（SGAPs）。淀粉合成相关蛋白是正常和糯玉米淀粉中的主要sgap。在水稻淀粉中，sgap以种子贮藏蛋白为主。在普通玉米淀粉和普通水稻淀粉中，sgap呈同心圆环状分布，与颗粒结合淀粉合成酶有关。糯玉米淀粉的sgap主要发生在淀粉通道中，被鉴定为淀粉合成酶和淀粉分支酶。碱处理通过促进蛋白质溶解度和降解蛋白质结构来降低sgap。水热处理促进了碱的作用。碱处理后，淀粉合成相关蛋白占总SGAPs的比例显著增加，说明这些蛋白与淀粉颗粒紧密结合，具有更强的抗碱能力。本研究揭示了淀粉颗粒中sgap的异质性及其对碱性处理的响应。

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来源期刊

Biomacromolecules 化学-高分子科学

CiteScore

10.60

自引率

4.80%

发文量

417

审稿时长

1.6 months

期刊介绍： Biomacromolecules is a leading forum for the dissemination of cutting-edge research at the interface of polymer science and biology. Submissions to Biomacromolecules should contain strong elements of innovation in terms of macromolecular design, synthesis and characterization, or in the application of polymer materials to biology and medicine. Topics covered by Biomacromolecules include, but are not exclusively limited to: sustainable polymers, polymers based on natural and renewable resources, degradable polymers, polymer conjugates, polymeric drugs, polymers in biocatalysis, biomacromolecular assembly, biomimetic polymers, polymer-biomineral hybrids, biomimetic-polymer processing, polymer recycling, bioactive polymer surfaces, original polymer design for biomedical applications such as immunotherapy, drug delivery, gene delivery, antimicrobial applications, diagnostic imaging and biosensing, polymers in tissue engineering and regenerative medicine, polymeric scaffolds and hydrogels for cell culture and delivery.