Correction to “Comparison of DNA Extraction Methods for the Detection of Canned Tuna Species with DNA Mini-Barcoding”

Abstract

A. J. Emmi, B. Fatusin, and R. S. Hellberg, “Comparison of DNA Extraction Methods for the Detection of Canned Tuna Species with DNA Mini-Barcoding,” Journal of Food Quality 2023 (2023): 7121260, https://doi.org/10.1155/2023/7121260.

In the article, there are errors in Section 2.3:

“The reaction mixture for canned tuna fish samples included the following: 11.2 mL sterile H2O, 17.5 mL Qiagen HotStarTaq Plus Master Mix (2X), 0.7 mL each of one 10-μM forward primer (CR_F) and two 10-μM reverse primers (CR_R1 and CR_R2) (Mitchell and Hellberg, 2016), and 4.2 mL of DNA template, for a total reaction volume of 35 μL.”

Should read:

“The reaction mixture for canned tuna fish samples included the following: 11.2 μL sterile H2O, 17.5 μL Qiagen HotStarTaq Plus Master Mix (2X), 0.7 μL each of one 10-μM forward primer (CR_F) and two 10-μM reverse primers (CR_R1 and CR_R2) (Mitchell and Hellberg, 2016), and 4.2 μL of DNA template, for a total reaction volume of 35 μL.”

“Precast 2.0% E-Gels with ethidium bromide (Invitrogen, Carlsbad, CA, USA) were used to confirm DNA amplification, with 20 mL PCR product loaded into each sample lane.”

Should read:

“Precast 2.0% E-Gels with ethidium bromide (Invitrogen, Carlsbad, CA, USA) were used to confirm DNA amplification, with 20 μL PCR product loaded into each sample lane.”

Lastly, there are errors in Section 3.4:

“Legally, the term “light tuna” applies to tuna with a Munsell value not greater than 5.3, while the term “white tuna” is reserved specifically for albacore and must not exceed a Munsell value of 6.3 (21 CFR 161.190 canned tuna). The presence of albacore tuna in a sample labeled as “light tuna” was also reported by Mitchell and Hellberg [6]. Both instances of mislabeling identified in the current study could cause potential harm to at-risk groups, such as women and children, because of differences in mercury levels between tuna species.”

Should read:

“Section 3.4: Legally, the term “light tuna” applies to tuna with a Munsell value not darker than 5.3, while the term “white tuna” is reserved specifically for albacore and must not be darker than a Munsell value of 6.3 (21 CFR 161.190 canned tuna). The presence of albacore tuna in a sample labeled as “light tuna” was also reported by Mitchell and Hellberg [6]. Both labeling concerns identified in the current study could cause potential harm to at-risk groups, such as women and children, because of differences in mercury levels between tuna species.”

We apologize for these errors.