Development, characterization, and in vitro cytotoxicity of resveratrol-loaded lipid polymer hybrid nanocarriers (LPHN) in glioblastoma multiforme cells

Abstract

The current research work aimed to develop, characterize, and assess in vitro cytotoxicity of resveratrol (RSV)-loaded lipid polymer hybrid nanocarriers (LPHN) in glioblastoma multiforme cells. RSV-LPHN was developed by central composite design (CCD). The developed RSV-LPHN were characterized by particle size (PS), polydispersity index (PDI), zeta (ζ) potential, % entrapment efficiency, and in vitro drug release. The surface morphology was studied using SEM and TEM techniques. FTIR, DSC, proton nuclear magnetic resonance (¹H NMR) study, and X-ray diffraction studies (XRD) were also performed. The EE was found in the range of 62–70%, while PDI was between 0.126 and 0.264 exhibiting a monodispersed nature. Zeta potential was found between −15 and −46 mV. Surface morphology through TEM revealed smoothH needle-like structures of the RSV-LPHN. The optimized formulation exhibited nearly 87% drug release over 24 h revealing excellent sustained release behavior in comparison to pure drug. Excellent drug and excipient compatibility was observed in the FTIR study. DSC and ¹H NMR study revealed proper encapsulation of RSV in the lipidic matrix. XRD study confirmed the amorphous nature of the RSV-LPHN. Cellular uptake studies performed in U87 cells showed uptake of 162.26% from RSV-LPHN to control (99.38%) demonstrating maximum cellular internalization. Different concentrations of RSV showed a high percent of inhibition and anticancer activity against the glioblastoma cancer cell line as compared to 5FU. The present study authenticates the potential of RSV-LPHN for efficient management of glioblastoma multiforme.