Synthesis of New macromer Based on HEMA-TA-PEG for Preparation of Bio-Based Hydrogels for Regenerative Medicine Applications

IF 5 3区工程技术 Q2 ENGINEERING, ENVIRONMENTAL

Journal of Polymers and the Environment Pub Date : 2025-05-17 DOI:10.1007/s10924-025-03584-7

Hossein Rayat Pisheh, Alireza Sabzevari, Mojtaba Ansari, Kourosh Kabiri, Hossein Eslami, Mohammad Kohestanian

{"title":"Synthesis of New macromer Based on HEMA-TA-PEG for Preparation of Bio-Based Hydrogels for Regenerative Medicine Applications","authors":"Hossein Rayat Pisheh, Alireza Sabzevari, Mojtaba Ansari, Kourosh Kabiri, Hossein Eslami, Mohammad Kohestanian","doi":"10.1007/s10924-025-03584-7","DOIUrl":null,"url":null,"abstract":"<div><p>In this study, bio-based hydrogels were designed and synthesized using the HEMA-TA-PEG macromer. The chemical structure of the macromer was confirmed through FTIR and <sup>1</sup>H-NMR analyses. Hydrogels with varying crosslinker (GPTMS) content were fabricated to investigate the impact of GPTMS on swelling, gel content, and mechanical properties. As the GPTMS content increased, the swelling behavior of the hydrogels decreased, whereas the gel content increased. Mechanical testing revealed a significant increase in tensile strength, with stress rising from 517 kPa to 786 kPa and the tensile modulus reaching 1.03 MPa as the GPTMS content increased from 0.25 g to 1 g. The prepared hydrogels exhibited excellent cytocompatibility, with significantly higher cell viability than the control over time (24, 48, and 72 h). Furthermore, cell adhesion in the hydrogels reached 93% at 48 h, a significant increase compared to the 85% observed in the control. These findings highlight the potential of bio-based hydrogels for applications in regenerative medicine.</p><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":659,"journal":{"name":"Journal of Polymers and the Environment","volume":"33 7","pages":"3076 - 3091"},"PeriodicalIF":5.0000,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Polymers and the Environment","FirstCategoryId":"5","ListUrlMain":"https://link.springer.com/article/10.1007/s10924-025-03584-7","RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENGINEERING, ENVIRONMENTAL","Score":null,"Total":0}

引用次数: 0

Abstract

In this study, bio-based hydrogels were designed and synthesized using the HEMA-TA-PEG macromer. The chemical structure of the macromer was confirmed through FTIR and ¹H-NMR analyses. Hydrogels with varying crosslinker (GPTMS) content were fabricated to investigate the impact of GPTMS on swelling, gel content, and mechanical properties. As the GPTMS content increased, the swelling behavior of the hydrogels decreased, whereas the gel content increased. Mechanical testing revealed a significant increase in tensile strength, with stress rising from 517 kPa to 786 kPa and the tensile modulus reaching 1.03 MPa as the GPTMS content increased from 0.25 g to 1 g. The prepared hydrogels exhibited excellent cytocompatibility, with significantly higher cell viability than the control over time (24, 48, and 72 h). Furthermore, cell adhesion in the hydrogels reached 93% at 48 h, a significant increase compared to the 85% observed in the control. These findings highlight the potential of bio-based hydrogels for applications in regenerative medicine.

Abstract Image

查看原文本刊更多论文

基于HEMA-TA-PEG的新型高分子材料制备再生医学用生物基水凝胶

本研究采用HEMA-TA-PEG大分子设计合成了生物基水凝胶。通过红外光谱（FTIR）和核磁共振核谱（1H-NMR）分析证实了该大分子的化学结构。制备了不同交联剂（GPTMS）含量的水凝胶，以研究GPTMS对膨胀、凝胶含量和力学性能的影响。随着GPTMS含量的增加，水凝胶的溶胀性降低，而凝胶含量增加。力学试验表明，当GPTMS含量从0.25 g增加到1 g时，抗拉强度显著增加，应力从517 kPa增加到786 kPa，拉伸模量达到1.03 MPa。制备的水凝胶表现出良好的细胞相容性，随着时间的推移（24、48和72 h），细胞活力明显高于对照组。此外，48 h时，细胞在水凝胶中的粘附率达到93%，与对照组的85%相比有显著提高。这些发现突出了生物基水凝胶在再生医学中的应用潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Polymers and the Environment 工程技术-高分子科学

CiteScore

9.50

自引率

7.50%

发文量

297

审稿时长

9 months

期刊介绍： The Journal of Polymers and the Environment fills the need for an international forum in this diverse and rapidly expanding field. The journal serves a crucial role for the publication of information from a wide range of disciplines and is a central outlet for the publication of high-quality peer-reviewed original papers, review articles and short communications. The journal is intentionally interdisciplinary in regard to contributions and covers the following subjects - polymers, environmentally degradable polymers, and degradation pathways: biological, photochemical, oxidative and hydrolytic; new environmental materials: derived by chemical and biosynthetic routes; environmental blends and composites; developments in processing and reactive processing of environmental polymers; characterization of environmental materials: mechanical, physical, thermal, rheological, morphological, and others; recyclable polymers and plastics recycling environmental testing: in-laboratory simulations, outdoor exposures, and standardization of methodologies; environmental fate: end products and intermediates of biodegradation; microbiology and enzymology of polymer biodegradation; solid-waste management and public legislation specific to environmental polymers; and other related topics.