Optimization of Culture Conditions for the Solubilization of Silicate Minerals by Bacillus spp. Using Response Surface Methodology

IF 3.3 3区材料科学 Q3 CHEMISTRY, PHYSICAL

Silicon Pub Date : 2025-06-11 DOI:10.1007/s12633-025-03358-9

Gajendiran Manimaran, Selvi Duraisamy, Anandham Rangasamy, Thiyageshwari Subramanium, Senthil Alagarsamy, Prabhaharan James, Deepana Perumal, Jegan Periakaruppan

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引用次数: 0

Abstract

Silicon is an essential mineral that plays a crucial role in increasing plant growth, improving crop yields, and imparting resilience against environmental stresses. This study explored the silicate solubilization potential of Achromobacter sp. L1C9T2, Bacillus altitudinis L3C3T2, Bacillus safensis L5C13T, Bacillus altitudinis SSB4, and Priestia aryabhattai KSBN2K7 using three silicate minerals, magnesium trisilicate (MGT), quartz (QT), and bentonite (BT). Both qualitative and quantitative assessments revealed that the maximum silicate solubilization was exhibited by Bacillus altitudinis SSB4 (87.71%) and Priestia aryabhattai KSBN2K7 (85.65%) from MGT and QT. Silicate solubilization was negatively correlated (-0.715) with decreasing pH after 24 h, which was attributed to organic acids, as confirmed by GC‒MS analysis. The optimization of culture conditions, including pH, temperature, and carbon sources, revealed that a pH of 7, an incubation temperature of 30 °C, and 1% dextrose enhanced silicate solubilization. The structural alteration of silicates induced by bacterial activity was validated through FT-IR spectroscopy. Statistical modeling using response surface methodology revealed optimal conditions for maximizing silicate solubilization, with R² values of 0.9941 and 0.9304 for magnesium trisilicate and 0.9855 and 0.9675 for quartz, and inoculation with SSB4 and KSBN2K7 indicated strong predictive accuracy. These results highlight the promising use of Bacillus altitudinis SSB4 and Priestia aryabhattai KSBN2K7 as potent silicate-solubilizing bioinoculants to increase plant growth and agricultural productivity.

Graphical Abstract

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响应面法优化芽孢杆菌对硅酸盐矿物增溶的培养条件

硅是一种重要的矿物质，在促进植物生长、提高作物产量和增强对环境压力的抵抗力方面起着至关重要的作用。本研究利用三硅酸镁（MGT）、石英（QT）和膨润土（BT）三种硅酸盐矿物，探讨了无色杆菌sp. L1C9T2、高原芽孢杆菌L3C3T2、萨氏芽孢杆菌L5C13T、高原芽孢杆菌SSB4和Priestia aryabhattai KSBN2K7的硅酸盐增溶潜力。定性和定量评价均表明，MGT和QT试验中，Bacillus altitinis SSB4（87.71%）和Priestia aryabhattai KSBN2K7（85.65%）的硅酸盐增溶作用最大，且24 h后，硅酸盐增溶作用与pH降低呈负相关（-0.715），GC-MS分析证实了这是有机酸作用所致。优化培养条件，包括pH、温度和碳源，发现pH为7，培养温度为30℃，1%葡萄糖增强了硅酸盐的增溶作用。通过傅里叶变换红外光谱验证了细菌活性引起的硅酸盐结构变化。利用响应面法建立的统计模型显示，三硅酸镁的R2分别为0.9941和0.9304，石英的R2分别为0.9855和0.9675，接种SSB4和KSBN2K7具有较强的预测精度。这些结果突出了高海拔芽孢杆菌SSB4和Priestia aryabhattai KSBN2K7作为有效的硅酸盐溶解生物接种剂在促进植物生长和农业生产力方面的应用前景。图形抽象

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来源期刊

Silicon CHEMISTRY, PHYSICAL-MATERIALS SCIENCE, MULTIDISCIPLINARY

CiteScore

5.90

自引率

20.60%

发文量

685

审稿时长

>12 weeks

期刊介绍： The journal Silicon is intended to serve all those involved in studying the role of silicon as an enabling element in materials science. There are no restrictions on disciplinary boundaries provided the focus is on silicon-based materials or adds significantly to the understanding of such materials. Accordingly, such contributions are welcome in the areas of inorganic and organic chemistry, physics, biology, engineering, nanoscience, environmental science, electronics and optoelectronics, and modeling and theory. Relevant silicon-based materials include, but are not limited to, semiconductors, polymers, composites, ceramics, glasses, coatings, resins, composites, small molecules, and thin films.