Prevalence and genetic characteristics of antimicrobial-resistant Escherichia coli in wild birds in Japan.

IF 1.1 4区农林科学 Q3 VETERINARY SCIENCES

Journal of Veterinary Medical Science Pub Date : 2025-09-23 DOI:10.1292/jvms.25-0336

Tetsuo Asai, Michiyo Sugiyama, Tomoya Morimoto, Akiko Sudo, Junji Moribe, Masaru Usui

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引用次数: 0

Abstract

Antimicrobial-resistant bacteria in wild animals are a multisectoral concern worldwide. In this study, we examined fecal samples collected from wild birds in two prefectures of Japan between 2021 and 2024 using both deoxycholate-hydrogen sulfide-lactose (DHL) agar media and antimicrobial-containing DHL agar media. Of the 252 fecal samples from 11 bird species, 159 E. coli were isolated from 70 samples (27.8%) of six species, and resistance was found in 4.4% of isolates from DHL media. Using nalidixic acid (NAL)- and cefotaxime (CTX)-containing media, NAL-resistant isolates were isolated from great cormorants and spot-billed ducks, and an extended-spectrum β-lactamase (ESBL)/AmpC β-lactamase producer was isolated from great cormorants. Next-generation sequencing analysis of 19 E. coli isolates exhibiting resistance to NAL and/or CTX indicated diverse genotypes. Genetic analysis indicated that amino acid substitution of quinolone-resistance-determining-regions and qnrS were responsible for NAL resistance, and CTX-Ms (bla_CTX-M-14 in three isolates, and bla_CTX-M-15 and bla_CTX-M-55 in each) and AmpC β-lactamase (bla_MOX-4 and bla_CMY-2 in each) were responsible for CTX resistance. Despite the presence of certain resistant strains, the overall prevalence of antimicrobial-resistant E. coli remains low in wild birds, suggesting limited environmental exposure to antimicrobials and antimicrobial resistant bacteria.

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日本野禽耐药大肠杆菌的流行及遗传特征。

野生动物中的抗微生物细菌是全世界多部门关注的问题。在这项研究中，我们使用脱氧胆碱-硫化氢-乳糖（DHL）琼脂培养基和含抗菌剂的DHL琼脂培养基检查了2021年至2024年间从日本两个县收集的野生鸟类粪便样本。在11种鸟类的252份粪便样本中，从6种鸟类70份样本中分离出159株大肠杆菌（27.8%），从DHL培养基中分离出耐药菌株的比例为4.4%。采用含钠啶酸（NAL）和头孢噻肟（CTX）的培养基，从大鸬鹚和斑嘴鸭中分离到NAL耐药菌株，并从大鸬鹚中分离到广谱β-内酰胺酶(ESBL)/AmpC β-内酰胺酶产生物。下一代测序分析显示，19株对NAL和/或CTX耐药的大肠杆菌菌株具有不同的基因型。遗传分析表明，喹诺酮类耐药决定区和qnrS的氨基酸取代是NAL耐药的主要原因，CTX- ms（3株为blaCTX-M-14，各为blaCTX-M-15和blaCTX-M-55）和AmpC β-内酰胺酶（各为blaMOX-4和blaCMY-2）是CTX耐药的主要原因。尽管存在某些耐药菌株，但抗菌素耐药大肠杆菌在野生鸟类中的总体流行率仍然很低，这表明抗菌素和抗菌素耐药细菌的环境暴露有限。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Veterinary Medical Science 农林科学-兽医学

CiteScore

2.30

自引率

8.30%

发文量

230

审稿时长

9-18 weeks

期刊介绍： JVMS is a peer-reviewed journal and publishes a variety of papers on veterinary science from basic research to applied science and clinical research. JVMS is published monthly and consists of twelve issues per year. Papers are from the areas of anatomy, physiology, pharmacology, toxicology, pathology, immunology, microbiology, virology, parasitology, internal medicine, surgery, clinical pathology, theriogenology, avian disease, public health, ethology, and laboratory animal science. Although JVMS has played a role in publishing the scientific achievements of Japanese researchers and clinicians for many years, it now also accepts papers submitted from all over the world.