Protective effect of quercetin liposome on acute low dose diazinon-induced oxidative stress and neurobehavioral disorders by affecting serotonin metabolite in mature male rats.

Abstract

Diazinon (DZN) is a widely used organophosphate. We studied the effect of quercetin pegylated liposome (QPEGL) on acute low dose DZN-induced oxidative stress and behavioral disorders through monitoring brain serotonin metabolite 5-hydroxyindoleacetic acid (5-HIAA) in mature male rats. Animals were treated in two control groups that received a single dose of normal saline and dimethyl sulfoxide, and four groups that received a single dose of DZN 10.00 mg kg^-1 (DZN), DZN 10.00 mg kg + quercetin 20.00 mg kg^-1, DZN 10.00 mg kg^-1 + PEGL 20.00 mg kg^-1, DZN 10.00 mg kg^-1 + QPEGL 20.00 mg kg^-1 (QPEGL), respectively. Performances of the rats were investigated by the open field and elevated plus maze tests. Twenty-four hr after the treatments, animals' brains were harvested and frozen at - 80.00 ˚C. Brain tissues 5-HIAA level was determined by the enzyme-linked immunosorbent assay. Furthermore, malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GPx) levels were determined for oxidative stress analysis. The motor activity was significantly reduced in the DZN group compared to the control group following increased anxiety-like behavior and ameliorated by QPEGL. Moreover, 5-HIAA and MDA levels notably increased in the DZN group compared to the control group and significantly decreased in the QPEGL group compared to the DZN group. The SOD and GPx contents were not significantly changed in the DZN group compared to the control; although, these parameters improved after treatment with QPEGL. Acute low dose DZN exposure resulted in lipid peroxidation and elevated levels of the serotonin metabolite (5-HIAA), leading to neurobehavioral disorders, such as anxiety-like behavior and impaired motor activity, which were alleviated by QPEGL.