The Value of FilmArray Pneumonia Panel plus in Managing Nosocomial Respiratory Infections in Hospitalized Patients: Cross-sectional study.

Q2 Medicine

Clinica Terapeutica Pub Date : 2025-09-01 DOI:10.7417/CT.2025.5276

Omima Sayed Mohammed, Abdulmabod Omar, Mohammad AbdElhameed Alwaseef, Hatem Mohamed Newishy, Medhat Ali Salah Abd Elghaffar, Zeinab Adawy, Mahmoud Ahmed Arfa, Tarek Abualkasem Abualwafa Mohammed, Mohamed Abd Elrahman Abd Elkader Elbakery, Usama Abdalla, Hanem Mohamed Badawy, Mohammad Abd Elhalem Abd El Razik, Huda Ali Abdalrahim Alzubir, Fawzia A Sharaf, Bothayna I Saleh, Abdullah Mustafa Ibrahim Gaafar, Bassam Abdellatif Mahmoud Elsayed, Esam M Shawky, Mohamed Mahmoud Abdelfattah Abdelrahman, Mai Mosad Morsy, Mohamed Mounir Ahmed Rizk, Nahla Mohammed Eldeeb, Ahmed Rizk Elkholy, Wageih Salem ElNaghy, Ola A Elshora, Hossam M Farid El-Zamek, Ahmed R I Ali, Mohamed Basiouny Yahia, Fatma Ibrahim Ismail Mayah, Ahmed M Ewis, Eid Mohammed, Ahmed Atef Ganna, Esraa El-Sayed AbdelMaksoud Elmahdy

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引用次数: 0

Abstract

Background: The traditional reliance on slow culture-based diagnostics often leads to delays in targeted therapy and the overuse of broad-spectrum antibiotics, aggravating the problem of antimicrobial resistance.

Aim: To compare the BioFire, FilmArray Pneumonia Panel plus (FA-PneumobioMérieux) assay with a conventional diagnostic tools, including culture and VITEK 2 (bioMérieux), to identify microorganisms in critically ill patients (severe pulmonary infection) to get the best results within a short period.

Methods: An observational, comparative cross-sectional, hospital-based investigation was carried out at the microbiology unit of the clinical pathology department of Alazhar University hospitals. Lower respiratory tract samples were collected from patients with hospital-acquired pneumonia and ICU admissions. Conventional culture was carried out for all specimens. Susceptibility testing was performed on all specimens using the VITEK 2 system (bioMérieux). The BioFire FA-Pneumo assay was conducted according to the manufacturer's instructions.

Results: Out of 60 patients with pneumonia 90% of specimens were sputum samples. For all examined pathogens, there was no statistically significant difference between the detection rates of Multiplex PCR and Vitek methods (P-value >0.05). The most prevalent resistant gene detected by Multiplex PCR was gene (blaNDM-1 ), found in 23.3% (n=14) of the samples, followed by OXA-48-like, which was found in 15% (n=9) of the samples. The detection of CTX-, CTX-M, KPC, mecA/C, and MREJ, VIM resistant genes were at lower frequencies.

Conclusion: The FilmArray Pneumonia Panel Plus (FA-Pneumo) is a rapid molecular diagnostic tool designed to overcome the limitations of conventional methods by quickly identifying a broad range of pathogens and resistance genes. The capability of FA-Pneumo to rapidly identify both pathogens and key resistance genes provides a significant advantage for clinical decision-making.

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FilmArray肺炎面板加在住院患者院内呼吸道感染管理中的价值：横断面研究。

背景：传统上依赖缓慢的基于培养的诊断常常导致靶向治疗的延误和广谱抗生素的过度使用，加剧了抗菌素耐药性问题。目的：比较BioFire， FilmArray Pneumonia Panel plus （fa - pneumbiomsamrieux）检测与传统诊断工具（包括培养和VITEK 2 (biomsamrieux)）在危重患者（严重肺部感染）中鉴定微生物的效果，以在短时间内获得最佳结果。方法：在阿拉资哈尔大学附属医院临床病理科微生物科进行观察性、横断面比较、以医院为基础的调查。收集医院获得性肺炎和ICU住院患者的下呼吸道样本。所有标本均进行常规培养。使用VITEK 2系统（biomrieux）对所有标本进行药敏试验。BioFire FA-Pneumo试验按照制造商的说明进行。结果：60例肺炎患者中痰液标本占90%。对所有检出的病原菌，Multiplex PCR与Vitek法的检出率比较，差异无统计学意义（p值0.05）。多重PCR检出最多的耐药基因是blaNDM-1基因，占23.3% (n=14)，其次是oxa -48样基因，占15% （n=9）。CTX-、CTX- m、KPC、mecA/C和MREJ、VIM耐药基因检测频率较低。结论：FilmArray Pneumonia Panel Plus （FA-Pneumo）是一种快速分子诊断工具，旨在通过快速识别广泛的病原体和耐药基因，克服传统方法的局限性。FA-Pneumo快速识别病原体和关键耐药基因的能力为临床决策提供了显着优势。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinica Terapeutica PHARMACOLOGY & PHARMACY-

CiteScore

2.50

自引率

0.00%

发文量

124

审稿时长

6-12 weeks

期刊介绍： La Clinica Terapeutica è una rivista di Clinica e Terapia in Medicina e Chirurgia, fondata nel 1951 dal Prof. Mariano Messini (1901-1980), Direttore dell''Istituto di Idrologia Medica dell''Università di Roma “La Sapienza”. La rivista è pubblicata come “periodico bimestrale” dalla Società Editrice Universo, casa editrice fondata nel 1945 dal Comm. Luigi Pellino. La Clinica Terapeutica è indicizzata su MEDLINE, INDEX MEDICUS, EMBASE/Excerpta Medica.