Lacticaseibacillus rhamnosus and Limosilactobacillus reuteri probiotics modulate inflammation, attenuating chronic stress-induced liver injury and behavior in rats.

IF 3.8 3区医学 Q2 NUTRITION & DIETETICS

Journal of Nutrition Pub Date : 2025-09-22 DOI:10.1016/j.tjnut.2025.09.011

Lívia Bruni de Souza, Ana Beatriz P Brandão, Fabiana G Ferreira, Vinicius Guzzoni, Raquel C M F Albuquerque, Lys A Mendes, Rodrigo Yokota, Fernando G Porto, Dulce E Casarini, Flavio Aimbire, Nilsa R Damaceno-Rodrigues, Nádia Bertoncello, Karina R Casali, Tatiana S Cunha

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引用次数: 0

Abstract

Background: Chronic stress disrupts systemic homeostas and contributes to metabolic, psychiatric, and inflammatory disorders, often involving alterations in gut microbiota. Probiotics such as Lacticaseibacillus rhamnosus (L. rhamnosus) and Limosilactobacillus reuteri (L. reuteri) possess anti-inflammatory properties, but their combined effects on liver health and cognitive function remain underexplored.

Objective: Investigate whether supplementation with L. rhamnosus and L. reuteri mitigates inflammation, liver damage, and behavioral changes induced by chronic stress.

Methods: Forty-seven male Wistar rats were assigned to four groups: Control (C, n=12), Stress (S, n=12), Control Probiotic (CP, n=11), and Stress Probiotic (SP, n=12). All animals received standard laboratory chow. A combined dose of L. rhamnosus and L. reuteri (1×10⁹ CFU/strain/day) was administered by gavage for 8 weeks. Groups S and SP were subjected to a chronic mild unpredictable stress protocol during weeks 3-5, including stressors such as food/water deprivation, restraint, and light/dark cycle inversion. Behavioral, biochemical, inflammatory, hepatic, metabolic, and physiological parameters were assessed.

Results: Probiotic supplementation significantly restored body weight gain (SP: 123.5±17.5% vs. S: 98.2±12.2%) and sucrose preference (SP: 19.96±0.31% vs. S: 14.48±4.16%). SP rats showed reduced levels of corticosterone (SP: 65.2±31.2 ng/mL; S: 188±112.6 ng/mL), noradrenaline (SP: 243±163.6 pg/mL; S: 887.6±476 pg/mL), and dopamine (SP: 318.6±169 pg/mL; S: 582.8±271 pg/mL). Supplementation also decreased ALT levels (SP: 131.6±9.4 U/L vs. S: 147.6±7.5 U/L) and hepatocyte nuclear area (SP: 11.35±1.5 μm² vs. S: 12.63 ± 1.7 μm²). In circulating analyses, probiotics modulated inflammation by reducing IL-1β (SP: 115±43 pg/mg; S: 343±64 pg/mg) and IL-17 (SP: 356.5±93 pg/mg; S: 674±48 pg/mg), while increasing IL-10 levels (SP: 196.5±11 pg/mg; S: 65±28.6 pg/mg) (all p < 0.05).

Conclusion: L. rhamnosus and L. reuteri supplementation attenuated the metabolic, hormonal, inflammatory, and behavioral effects of chronic stress and preserved liver integrity in rats.

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鼠李糖乳杆菌和罗伊氏乳酸杆菌益生菌调节炎症，减轻慢性应激性肝损伤和大鼠行为。

背景：慢性应激破坏系统内稳态，导致代谢、精神和炎症性疾病，通常涉及肠道微生物群的改变。益生菌如鼠李糖乳杆菌（L. rhamnosus）和罗伊氏乳酸杆菌（L. reuteri）具有抗炎特性，但它们对肝脏健康和认知功能的综合影响仍未得到充分研究。目的：探讨鼠李糖乳杆菌和罗伊氏乳杆菌是否能减轻慢性应激引起的炎症、肝损伤和行为改变。方法：雄性Wistar大鼠47只，随机分为4组：对照组（C, n=12）、应激组（S, n=12）、对照组益生菌组（CP, n=11）和应激益生菌组（SP, n=12）。所有的动物都吃标准的实验室食物。鼠李糖乳杆菌和罗伊氏乳杆菌联合给药（1×109 CFU/株/天）灌胃8周。S组和SP组在第3-5周接受慢性轻度不可预测的应激方案，包括食物/水剥夺、约束和光/暗周期反转等应激源。评估行为、生化、炎症、肝脏、代谢和生理参数。结果：补充益生菌可显著恢复体重增加（SP: 123.5±17.5%,S: 98.2±12.2%）和蔗糖偏好（SP: 19.96±0.31%,S: 14.48±4.16%）。SP大鼠皮质酮（SP: 65.2±31.2 ng/mL; S: 188±112.6 ng/mL）、去甲肾上腺素（SP: 243±163.6 pg/mL; S: 887.6±476 pg/mL）、多巴胺（SP: 318.6±169 pg/mL; S: 582.8±271 pg/mL）水平降低。ALT水平（SP: 131.6±9.4 U/L vs. S: 147.6±7.5 U/L）和肝细胞核面积（SP: 11.35±1.5 μm2 vs. S: 12.63±1.7 μm2）降低。在循环分析中，益生菌通过降低IL-1β （SP: 115±43 pg/mg; S: 343±64 pg/mg）和IL-17 (SP: 356.5±93 pg/mg; S: 674±48 pg/mg)，增加IL-10水平（SP: 196.5±11 pg/mg; S: 65±28.6 pg/mg）来调节炎症（均p < 0.05）。结论：鼠李糖乳杆菌和罗伊氏乳杆菌的补充减轻了慢性应激对大鼠的代谢、激素、炎症和行为的影响，并保持了肝脏的完整性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Nutrition 医学-营养学

CiteScore

7.60

自引率

4.80%

发文量

260

审稿时长

39 days

期刊介绍： The Journal of Nutrition (JN/J Nutr) publishes peer-reviewed original research papers covering all aspects of experimental nutrition in humans and other animal species; special articles such as reviews and biographies of prominent nutrition scientists; and issues, opinions, and commentaries on controversial issues in nutrition. Supplements are frequently published to provide extended discussion of topics of special interest.