Assessment of the relationship between gut microbiota, inflammatory markers, and colorectal cancer.

IF 4.8 2区医学 Q2 IMMUNOLOGY

Frontiers in Cellular and Infection Microbiology Pub Date : 2025-09-09 eCollection Date: 2025-01-01 DOI:10.3389/fcimb.2025.1604651

Yan Cui

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引用次数: 0

Abstract

Objective: This study aims to evaluate the correlation between inflammation and gut microbiota characteristics in patients with colorectal cancer (CRC) through a retrospective study.

Methods: This cross-sectional, non-interventional study included a total of 200 subjects, of which 150 were colorectal cancer (CRC) patients and 50 were healthy individuals. The study retrospectively reviewed hospital and laboratory archives and records from 2015 to 2020. Gut microbiota was analyzed using 16S rRNA sequencing. Inflammatory markers, including C-reactive protein (CRP), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), and interleukin-beta (IL-1β), were measured using enzyme-linked immunosorbent assay (ELISA). Logistic regression analysis was used to evaluate the relationship between gut microbiota, inflammatory markers, and CRC.

Results: Subjects in the colorectal cancer (CRC) group exhibited a higher proportion of Firmicutes (47.2% vs. 39.0%). Levels of both Firmicutes and Proteobacteria were significantly elevated in the CRC group, while Bacteroidetes levels were lower. Additionally, elevated levels of inflammatory markers were observed in the CRC group, including C-reactive protein (CRP: 9.8 mg/L vs. 4.1 mg/L, P<0.01), interleukin-6 (IL-6: 14.5 pg/mL vs. 6.2 pg/mL, P<0.01), tumor necrosis factor-alpha (TNF-α: 9.2 pg/mL vs. 4.3 pg/mL, P<0.01), and interleukin-1β (IL-1β: 5.8 pg/mL vs. 3.6 pg/mL, P<0.01). Multivariate analysis showed that higher levels of Firmicutes (OR=2.5, 95% CI: 1.4-4.5, P<0.01) and Proteobacteria (OR=2.8, 95% CI: 1.6-4.9, P<0.01) were significantly associated with an increased risk of CRC. Elevated levels of CRP (OR=3.1, 95% CI: 1.8-5.3, P<0.01) and IL-6 (OR=3.4, 95% CI: 2.0-5.8, P<0.01) were also significantly associated with an increased risk of CRC.

Conclusion: There is a significant correlation between changes in gut microbiota composition and cytokine levels with the risk of colorectal cancer (CRC).

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评估肠道微生物群、炎症标志物和结直肠癌之间的关系。

目的：本研究旨在通过回顾性研究，评估结直肠癌（CRC）患者炎症与肠道菌群特征的相关性。方法：本横断面非介入性研究共纳入200例受试者，其中150例为结直肠癌患者，50例为健康人。该研究回顾性地回顾了2015年至2020年医院和实验室的档案和记录。采用16S rRNA测序分析肠道菌群。采用酶联免疫吸附试验（ELISA）检测炎症标志物，包括c反应蛋白（CRP）、白细胞介素-6 （IL-6）、肿瘤坏死因子-α (TNF-α)和白细胞介素- β (IL-1β)。采用Logistic回归分析来评估肠道微生物群、炎症标志物和结直肠癌之间的关系。结果：结直肠癌（CRC）组中厚壁菌门的比例更高（47.2%比39.0%）。CRC组中厚壁菌门和变形菌门水平均显著升高，而拟杆菌门水平较低。此外，在结直肠癌组中观察到炎症标志物水平升高，包括c反应蛋白（CRP: 9.8 mg/L vs. 4.1 mg/L, Pvs. 6.2 pg/mL, Pvs. 4.3 pg/mL, Pvs. 3.6 pg/mL, pv）。结论：肠道微生物群组成和细胞因子水平的变化与结直肠癌（CRC）的风险有显著相关性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Frontiers in Cellular and Infection Microbiology IMMUNOLOGY-MICROBIOLOGY

CiteScore

7.90

自引率

7.00%

发文量

1817

审稿时长

14 weeks

期刊介绍： Frontiers in Cellular and Infection Microbiology is a leading specialty journal, publishing rigorously peer-reviewed research across all pathogenic microorganisms and their interaction with their hosts. Chief Editor Yousef Abu Kwaik, University of Louisville is supported by an outstanding Editorial Board of international experts. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide. Frontiers in Cellular and Infection Microbiology includes research on bacteria, fungi, parasites, viruses, endosymbionts, prions and all microbial pathogens as well as the microbiota and its effect on health and disease in various hosts. The research approaches include molecular microbiology, cellular microbiology, gene regulation, proteomics, signal transduction, pathogenic evolution, genomics, structural biology, and virulence factors as well as model hosts. Areas of research to counteract infectious agents by the host include the host innate and adaptive immune responses as well as metabolic restrictions to various pathogenic microorganisms, vaccine design and development against various pathogenic microorganisms, and the mechanisms of antibiotic resistance and its countermeasures.