The role of IL-18BP in alleviating anxiety-like behaviors after traumatic brain injury in rats by modulating astrocytic pyroptosis in amygdala.

IF 1.8 4区医学 Q4 NEUROSCIENCES

Brain injury Pub Date : 2025-09-25 DOI:10.1080/02699052.2025.2560587

Dongxue Wu, Xue Li, Yali Xing, Yaxiong Li, Yan Li, Conghui Li, Yong Wang, Hui Li, Jianfeng Liu, Wenchao Zhang

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引用次数: 0

Abstract

Primary objective: This study investigates the effect and underlying mechanisms of IL-18 binding protein (IL-18BP) on anxiety-like behaviors in rats following traumatic brain injury (TBI).

Methods and procedures: Thirty-six Sprague-Dawley rats were divided into three groups: sham, TBI, and TBI+IL-18BP. Anxiety-like behaviors were evaluated using the open field and elevated plus maze tests. Immunofluorescence staining was performed to assess the number of neurons, activated astrocytes, and the proportion of astrocytes positive for NLRP3 and IL-18. IL-18 levels in serum and cerebrospinal fluid (CSF) were quantified via ELISA. Western blot analysis was conducted to measure the expression of NLRP3, cleaved caspase-1 (cl-caspase-1), and N-GSDMD in the amygdala.

Main outcomes and results: Thirty days post-TBI, both the TBI and TBI+IL-18BP groups exhibited increased anxiety-like behaviors, elevated IL-18 levels in serum and CSF, greater activation of astrocytes in the amygdala, a higher percentage of NLRP3 and IL-18-positive astrocytes, and upregulated expression of pyroptosis-related proteins compared to the sham group (p < 0.05). However, the TBI+IL-18BP group showed significant reductions in these parameters compared to the TBI group (p < 0.05).

Conclusions: IL-18BP appears to mitigate anxiety-like behaviors in TBI rats, likely through a mechanism that involves reducing astrocyte apoptosis.

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IL-18BP通过调节杏仁核星形细胞凋亡在大鼠创伤性脑损伤后焦虑样行为中的作用

主要目的：探讨IL-18结合蛋白（IL-18BP）对创伤性脑损伤（TBI）大鼠焦虑样行为的影响及其机制。方法和步骤：将36只sd大鼠分为假手术组、TBI组和TBI+IL-18BP组。焦虑样行为采用开阔场和高架加迷宫测试进行评估。免疫荧光染色评估神经元数量、活化星形胶质细胞数量以及NLRP3和IL-18阳性星形胶质细胞比例。ELISA法测定血清和脑脊液中IL-18水平。Western blot检测NLRP3、cleaved caspase-1 （cl-caspase-1）和N-GSDMD在杏仁核中的表达。主要结局和结果：与假手术组相比，TBI组和TBI+IL-18BP组均表现出焦虑样行为增加，血清和脑脊液中IL-18水平升高，杏仁核中星形胶质细胞活化程度更高，NLRP3和IL-18阳性星形胶质细胞比例更高，焦热相关蛋白表达上调（p p）。IL-18BP似乎可以减轻脑外伤大鼠的焦虑样行为，可能是通过减少星形胶质细胞凋亡的机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Brain injury 医学-康复医学

CiteScore

3.50

自引率

5.30%

发文量

148

审稿时长

12 months

期刊介绍： Brain Injury publishes critical information relating to research and clinical practice, adult and pediatric populations. The journal covers a full range of relevant topics relating to clinical, translational, and basic science research. Manuscripts address emergency and acute medical care, acute and post-acute rehabilitation, family and vocational issues, and long-term supports. Coverage includes assessment and interventions for functional, communication, neurological and psychological disorders.