Efficient methods of isolation and purification of extracellular vesicles

IF 11 2区材料科学 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY

Nano Convergence Pub Date : 2025-09-25 DOI:10.1186/s40580-025-00509-x

Taewoon Kim, Jong Wook Hong, Luke P. Lee

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引用次数: 0

Abstract

Living cells produce nanometer scales of extracellular vesicles (EVs) that have attracted considerable interest due to their transformative effects on diagnostics and therapies for cancer and other diseases. While significant advancements have been made in grasping the physical and chemical foundations of separation techniques for EVs, challenges must be overcome to ensure effective EV purification for diverse life sciences and clinical applications. This review highlights the most significant developments in efficient isolation and purification methods for EVs in transformative medicine. We examine the basic structure of exosomes and how to obtain specimens containing exosomes and EVs from various body fluids. We investigate the principles of physical, chemical, and biological isolation methods of EVs. We systematically evaluate different designs of microfluidics-based EV purification methods. We provide a comprehensive overview of the applications of exosomes in the life sciences and medicine. The precise engineering of EV isolation and purification generates a high yield and purity, offering practical solutions for translational medicine.

Graphical Abstract

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细胞外囊泡的有效分离纯化方法。

活细胞产生纳米级的细胞外囊泡（EVs），由于其在癌症和其他疾病的诊断和治疗方面的变革性作用而引起了相当大的兴趣。虽然在掌握电动汽车分离技术的物理和化学基础方面取得了重大进展，但要确保电动汽车在各种生命科学和临床应用中的有效纯化，还需要克服一些挑战。本文综述了转型医学中高效分离和纯化ev方法的最重要进展。我们研究了外泌体的基本结构以及如何从各种体液中获得含有外泌体和ev的标本。我们研究了ev的物理、化学和生物分离方法的原理。我们系统地评估了基于微流体的EV净化方法的不同设计。我们提供了外泌体在生命科学和医学应用的全面概述。精确的EV分离和纯化工程产生高产量和纯度，为转化医学提供实用的解决方案。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nano Convergence Engineering-General Engineering

CiteScore

15.90

自引率

2.60%

发文量

审稿时长

13 weeks

期刊介绍： Nano Convergence is an internationally recognized, peer-reviewed, and interdisciplinary journal designed to foster effective communication among scientists spanning diverse research areas closely aligned with nanoscience and nanotechnology. Dedicated to encouraging the convergence of technologies across the nano- to microscopic scale, the journal aims to unveil novel scientific domains and cultivate fresh research prospects. Operating on a single-blind peer-review system, Nano Convergence ensures transparency in the review process, with reviewers cognizant of authors' names and affiliations while maintaining anonymity in the feedback provided to authors.