Reactor identification and evaluation of Brucella abortus vaccination results for cattle in Malaka Regency, East Nusa Tenggara Province, Indonesia.

Abstract

Background: One disease that interferes with reproductive performance is brucellosis, which causes significant economic losses.

Aim: This study aimed to identify the reactor and evaluate the results of a Brucella abortus vaccination in the Malaka Regency.

Methods: This research method involves the first stage, which involves sampling serum from 100 cattle. Serum specimens are derived from blood taken from the jugular or coccygeal vein using a Vacutainer tube filled with silicone and left at room temperature until the blood is separated into serum and clot. Then, for serological tests in the form of the Rose Bengal test (RBT), if the RBT test is positive, followed by the complement fixation test (CFT) test, and the CFT test is also positive, the patient is designated as a brucellosis reactor. At the same time, if the CFT is positive in vaccinated cattle, the vaccination is considered successful, and if it is negative, it is necessary to reevaluate the immunization.

Result: The results of 100 samples showed that the RBT and CFT tests yielded two distinct outcomes: a positive RBT and a negative CFT test, indicating that no B. abortus reactors were detected.

Conclusion: Cattle in Malacca Regency were not found to be positive for the B. abortus reactor. Vaccination results against Brucella are not optimal, as indicated by the absence of an antibody response, as determined by RBT and CFT tests.