Biocompatibility and bioactivity of bioceramic endodontic sealer: NeoSealer Flo.

IF 2.6 3区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Applied Oral Science Pub Date : 2025-09-22 eCollection Date: 2025-01-01 DOI:10.1590/1678-7757-2025-0284

Evelin Carine Alves Silva, Jéssica Arielli Pradelli, Guilherme Ferreira da Silva, Paulo Sérgio Cerri, Mario Tanomaru-Filho, Juliane Maria Guerreiro-Tanomaru

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引用次数: 0

Abstract

Objective: Bioceramic materials are developed based on their biological properties and bioactive potential. NeoSealer Flo (NeoFlo, NuSmile, Houston) is a ready-to-use bioceramic endodontic sealer. The aim of this study was to evaluate the biocompatibility and bioactivity of NeoFlo compared to Bio-C Sealer (BC, Angelus) and AH Plus (AHP, Dentsply).

Methodology: The tissue reaction induced by materials in rat subcutaneous tissues was assessed at seven, 15-, 30-, and 60-days post-implantation (n=6/group). The number of inflammatory cells (ICs), fibroblasts, and osteocalcin (OCN)-labelled cells were recorded. Amorphous calcite was identified using the von Kossa method and polarized light. The data were evaluated using two-way ANOVA followed by Tukey's test, with a 5% significance level. OCN data were submitted to the Kruskal-Wallis test, with Dunn, Friedman, and Nemenyi post-hoc tests.

Results: NeoFlo capsules showed higher number of IC than BC and AHP (p<0.05) in all periods, with a reduction over time, and was considered moderate at 60 days. Moreover, significant reduction in the number of IC and an increase in the fibroblasts was accompanied by an increase in the amount of collagen in the capsules around all materials over time. Immunoexpression of OCN was only observed in the capsules of NeoFlo and BC, but the capsules of BC showed the highest values in all periods (p<0.05).

Conclusion: In the present study, NeoFlo showed lower biocompatibility than BC, however, NeoFlo shows bioactivity in connective tissue.

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生物陶瓷牙髓密封材料的生物相容性和生物活性：NeoSealer Flo。

目的：利用生物陶瓷材料的生物学特性和生物活性潜力开发生物陶瓷材料。NeoSealer Flo （NeoFlo, NuSmile, Houston）是一种即用型生物陶瓷牙髓密封剂。本研究的目的是比较NeoFlo与Bio-C Sealer （BC, Angelus）和AH Plus （AHP, Dentsply）的生物相容性和生物活性。方法：分别于植入后7天、15天、30天和60天（n=6/组）观察材料在大鼠皮下组织诱导的组织反应。记录炎症细胞（ICs）、成纤维细胞和骨钙素（OCN）标记细胞的数量。利用von Kossa法和偏振光对非晶态方解石进行了鉴定。数据采用双因素方差分析和Tukey检验进行评估，显著性水平为5%。OCN数据提交给Kruskal-Wallis测试，Dunn、Friedman和Nemenyi事后测试。结果：NeoFlo胶囊的IC数量高于BC和AHP (p)。结论：在本研究中，NeoFlo的生物相容性低于BC，但NeoFlo在结缔组织中具有生物活性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Applied Oral Science 医学-牙科与口腔外科

CiteScore

4.80

自引率

3.70%

发文量

审稿时长

4-8 weeks

期刊介绍： The Journal of Applied Oral Science is committed in publishing the scientific and technologic advances achieved by the dental community, according to the quality indicators and peer reviewed material, with the objective of assuring its acceptability at the local, regional, national and international levels. The primary goal of The Journal of Applied Oral Science is to publish the outcomes of original investigations as well as invited case reports and invited reviews in the field of Dentistry and related areas.